LOCUS       BBa_K1334025            1728 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG inducible E7 protein generator
ACCESSION   BBa_K1334025
VERSION     BBa_K1334025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     terminator      1223..1351
                     /label=BBa_B0015
     promoter        1360..1421
                     /label=BBa_K1334028
     misc_feature    1434..1437
                     /label=conserved
     RBS             1430..1441
                     /label=BBa_B0034
     CDS             1448..1591
                     /label=BBa_K117000
     stem_loop       1611..1654
                     /label=stem_loop
     misc_feature    1600..1679
                     /label=BBa_B0010
     terminator      1600..1679
                     /label=BBa_B0010
     stem_loop       1695..1714
                     /label=T7 TE
     misc_feature    1721..1721
                     /label=stop
     polyA_site      1715..1728
                     /label=polya
     misc_feature    1688..1728
                     /label=BBa_B0012
     terminator      1688..1728
                     /label=BBa_B0012
     terminator      1600..1728
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag atactagaga aagaggagaa
     1441 atactagatg aaaaaaataa cagggattat tttattgctt cttgcagcca ttattcttgc
     1501 tgcatgtcag gcaaactata tccgtgatgt tcagggcggg acagtatcac cgtcgtcaac
     1561 tgctgaactg accggagtgg aaacgcagta atactagagc caggcatcaa ataaaacgaa
     1621 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1681 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//