LOCUS       BBa_K1334026            2468 bp    DNA     linear       28-Sep-2024
DEFINITION  IPTG inducible E7 protein and GFP generator
ACCESSION   BBa_K1334026
VERSION     BBa_K1334026.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     terminator      1223..1351
                     /label=BBa_B0015
     misc_feature    1362..1378
                     /label=lac O1
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1401
                     /label=lac O1
     promoter        1402..1407
                     /label=-10
     misc_feature    1360..1413
                     /label=BBa_R0011
     promoter        1360..1413
                     /label=BBa_R0011
     RBS             1423..1435
                     /label=RBS-3\Weak
     RBS             1429..1432
                     /label=RBS
     misc_feature    1423..1435
                     /label=BBa_B0032
     RBS             1423..1435
                     /label=BBa_B0032
     CDS             1442..2161
                     /label=GFP protein
     CDS             1442..2161
                     /label=BBa_E0040
     misc_feature    2174..2177
                     /label=conserved
     RBS             2170..2181
                     /label=BBa_B0034
     CDS             2188..2331
                     /label=BBa_K117000
     stem_loop       2351..2394
                     /label=stem_loop
     misc_feature    2340..2419
                     /label=BBa_B0010
     terminator      2340..2419
                     /label=BBa_B0010
     stem_loop       2435..2454
                     /label=T7 TE
     misc_feature    2461..2461
                     /label=stop
     polyA_site      2455..2468
                     /label=polya
     misc_feature    2428..2468
                     /label=BBa_B0012
     terminator      2428..2468
                     /label=BBa_B0012
     terminator      2340..2468
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag agtcacacag gaaagtacta
     1441 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1501 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1561 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1621 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1681 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1741 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1801 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1861 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1921 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1981 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2041 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2101 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     2161 atactagaga aagaggagaa atactagatg aaaaaaataa cagggattat tttattgctt
     2221 cttgcagcca ttattcttgc tgcatgtcag gcaaactata tccgtgatgt tcagggcggg
     2281 acagtatcac cgtcgtcaac tgctgaactg accggagtgg aaacgcagta atactagagc
     2341 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2401 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2461 cgtttata
//