BBa_K1336000
1
BBa_K1336000
Azoreductase gene from Pseudomonas putida
2014-10-05T11:00:00Z
2015-05-08T01:09:58Z
From genomic sequence of Pseudomonas putida; AzoR gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
A gene (612bp) coding for a non-specific enzyme from Pseudomonas putida that is used to degrade a double nitrogen (N=N) azo bond, as found in azo dyes.
false
false
_1711_
0
20842
9
Not in stock
false
When designing PCR primers to change the AzoR gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there are 2 illegal PstI restriction sites within the native gene.
false
Yan-Kay Ho
BBa_K1336000_sequence
1
atgaaactgttgcacatcgattcgagcatcctgggcgacaattccgcctcgcgtcagctgagccgtgaagtggtcgaagcctggaaggctgcagacccaagcgttgaagtggtttatcgcgacctggctgccgatgccatcgcccacttctccgctgccaccctggtcgctgcaggcacccctgaagacgtacgcgacgcggcccaggctttcgaagccaagctgagcgccgagacgctcgaagagttcctggctgccgatgccgtggtgattggtgcgcccatgtacaacttcaccgtgccgacccagctcaaagcctggattgaccgcgtagccgtcgccggcaagaccttccgctacaccgaagccggcccgcaaggcctgtgcggcaacaagaaagtggtgctggtttccactgcgggtggcctgcacgctggccagccgactggcgccgggcatgaagacttcctgaaagtgttcctgggcttcatcgggattactgacctggaaatcgtccgggcccacggcctggcctatggcccggagcagcgcagccaggcgatcgatgctgcacaggcgcagattgccagcgagctgtttgcggctgcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z