BBa_K1336002
1
BBa_K1336002
Spore coat protein, laccase, from Bacillus subtilis
2014-10-05T11:00:00Z
2015-05-08T01:09:58Z
From genomic sequence of Bacillus subtilis; CotA gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
CotA gene (1542bp) coding for an enzyme from Bacillus subtilis that is used to degrade and decolourise azo dyes, through an oxidative process.
false
false
_1711_
0
20842
9
Not in stock
false
When designing PCR primers to change the CotA gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there are 2 illegal restriction sites within the native gene: EcoRI at position xxx, and XbaI at position xxx).
false
Yan-Kay Ho
BBa_K1336002_sequence
1
atgacacttgaaaaatttgtggatgctctcccaatcccagatacactaaagccagtacagcaatcaaaagaaaaaacatactacgaagtcaccatggaggaatgcactcatcagctccatcgcgatctccctccaacccgcctgtggggctacaacggcttatttccgggaccgaccattgaggttaaaagaaatgaaaacgtatatgtaaaatggatgaataaccttccttccacgcatttccttccgattgatcacaccattcatcacagtgacagccagcatgaagagcccgaggtaaagactgttgttcatttacacggcggcgtcacgccagatgatagtgacgggtatccggaggcttggttttccaaagactttgaacaaacaggaccttatttcaaaagagaggtttatcattatccaaaccagcagcgcggggctatattgtggtatcacgatcacgccatggcgctcaccaggctaaatgtctatgccggacttgtcggtgcatatatcattcatgacccaaaggaaaaacgcttaaaactgccttcagacgaatacgatgtgccgcttcttatcacagaccgcacgatcaatgaggatggttctttgttttatccgagcgcaccggaaaacccttctccgtcactgcctaatccttcaatcgttccggctttttgcggagaaaccatactcgtcaacgggaaggtatggccatacttggaagtcgagccaaggaaataccgattccgtgtcatcaacgcctccaatacaagaacctataacctgtcactcgataatggcggagattttattcagattggttcagatggagggctcctgccgcgatctgttaaactgaattctttcagccttgcgcctgctgaacgttacgatatcatcattgacttcacagcatatgaaggagaatcgatcattttggcaaacagcgcgggctgcggcggtgacgtcaatcctgaaacagatgcgaatatcatgcaattcagagtcacaaaaccattggcacaaaaagacgaaagcagaaagccgaagtacctcgcctcatacccttcggtacagcatgaaagaatacaaaacatcagaacgttaaaactggcaggcacccaggacgaatacggcagacccgtccttctgcttaataacaaacgctggcacgatcccgtcacagaaacaccaaaagtcggcacaactgaaatatggtccattatcaacccgacacgcggaacacatccgatccacctgcatctagtctccttccgtgtattagaccggcggccgtttgatatcgcccgttatcaagaaagcggggaattgtcctataccggtccggctgtcccgccgccgccaagtgaaaagggctggaaagacaccattcaagcgcatgcaggtgaagtcctgagaatcgcggcgacattcggtccgtacagcggacgatacgtatggcattgccatattctagagcatgaagactatgacatgatgagaccgatggatataactgatccccataaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z