BBa_K1336002 1 BBa_K1336002 Spore coat protein, laccase, from Bacillus subtilis 2014-10-05T11:00:00Z 2015-05-08T01:09:58Z From genomic sequence of Bacillus subtilis; CotA gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group. CotA gene (1542bp) coding for an enzyme from Bacillus subtilis that is used to degrade and decolourise azo dyes, through an oxidative process. false false _1711_ 0 20842 9 Not in stock false When designing PCR primers to change the CotA gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there are 2 illegal restriction sites within the native gene: EcoRI at position xxx, and XbaI at position xxx). false Yan-Kay Ho BBa_K1336002_sequence 1 atgacacttgaaaaatttgtggatgctctcccaatcccagatacactaaagccagtacagcaatcaaaagaaaaaacatactacgaagtcaccatggaggaatgcactcatcagctccatcgcgatctccctccaacccgcctgtggggctacaacggcttatttccgggaccgaccattgaggttaaaagaaatgaaaacgtatatgtaaaatggatgaataaccttccttccacgcatttccttccgattgatcacaccattcatcacagtgacagccagcatgaagagcccgaggtaaagactgttgttcatttacacggcggcgtcacgccagatgatagtgacgggtatccggaggcttggttttccaaagactttgaacaaacaggaccttatttcaaaagagaggtttatcattatccaaaccagcagcgcggggctatattgtggtatcacgatcacgccatggcgctcaccaggctaaatgtctatgccggacttgtcggtgcatatatcattcatgacccaaaggaaaaacgcttaaaactgccttcagacgaatacgatgtgccgcttcttatcacagaccgcacgatcaatgaggatggttctttgttttatccgagcgcaccggaaaacccttctccgtcactgcctaatccttcaatcgttccggctttttgcggagaaaccatactcgtcaacgggaaggtatggccatacttggaagtcgagccaaggaaataccgattccgtgtcatcaacgcctccaatacaagaacctataacctgtcactcgataatggcggagattttattcagattggttcagatggagggctcctgccgcgatctgttaaactgaattctttcagccttgcgcctgctgaacgttacgatatcatcattgacttcacagcatatgaaggagaatcgatcattttggcaaacagcgcgggctgcggcggtgacgtcaatcctgaaacagatgcgaatatcatgcaattcagagtcacaaaaccattggcacaaaaagacgaaagcagaaagccgaagtacctcgcctcatacccttcggtacagcatgaaagaatacaaaacatcagaacgttaaaactggcaggcacccaggacgaatacggcagacccgtccttctgcttaataacaaacgctggcacgatcccgtcacagaaacaccaaaagtcggcacaactgaaatatggtccattatcaacccgacacgcggaacacatccgatccacctgcatctagtctccttccgtgtattagaccggcggccgtttgatatcgcccgttatcaagaaagcggggaattgtcctataccggtccggctgtcccgccgccgccaagtgaaaagggctggaaagacaccattcaagcgcatgcaggtgaagtcctgagaatcgcggcgacattcggtccgtacagcggacgatacgtatggcattgccatattctagagcatgaagactatgacatgatgagaccgatggatataactgatccccataaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z