BBa_K1336004 1 BBa_K1336004 Dye-decolourising peroxidase from Pseudomonas putida 2014-10-05T11:00:00Z 2015-05-08T01:09:58Z From genomic sequence of Pseudomonas putida; PpDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group. PpDyP gene (861bp) from Pseudomonas putida is a relatively novel gene, but which appears to be able to oxidise a wide variety of substrates at high efficiency, like that of azo dyes, anthraquinones, phenolic compounds, manganese, or veratryl alcohol. false false _1711_ 0 20842 9 It's complicated false When designing PCR primers to change the PpDyP gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there is 1 illegal restriction site within the native gene: EcoRI at position 72. false Yan-Kay Ho BBa_K1336004_sequence 1 atgccgttccagcaaggtctgcttgccaccccggtgccggcccacgcccgtcacctgttcttcaccctgcagtcgcccgaggcgttgcctgccgcgctggatgccttgctgccgcaggtcgatggcaaacagttgctgctgggcgtaggcgcccccttggccaaggccctgggccgggaaataccaggcctgcgcccgttcccgctactggatgccgcggttgagaaccccagcacccaacacgccctgtggctgtggctgcgtggcgacgaacgtggcgacctgctgctgcgcacccaggccctggagcaggcgctggcacccgccctgagcctggccgacagcgttgatggcttcctgcaccggggcggccatgaccttaccggttatgaagacggcaccgaaaacccgacagacgaagaggcggtgcaagcggccattgccgcagatggctccagctttgccgcgttccagctgtggaagcacgacctgcaatacttcaagtcgctgccccaggcagaccaggacaacatcatcggccgtcgcttgagcgataacgaagaactcgacgacgcccccgcgtccgcgcacgtcaagcgcaccgcccaggaaagctttgaacccgaggcgttcatggtccgtcgctcggtatcctgggccgaccaacgcggcgccggcctggctttcgtcgccctgggcaagagtttcgaggcattcgaggtgcagctgcggcgcatgagtggcctggaagacggcatcatcgacgggctgtaccgcttcagccgcccgctgacggggggctactactggtgcccgccaatgagcgagacgggtgtcgatctaagccccttgctgcgggcctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z