LOCUS       BBa_K1336007            2895 bp    DNA     linear       26-Dec-2024
DEFINITION  LacI Expression Cassette with BsDyP gene
ACCESSION   BBa_K1336007
VERSION     BBa_K1336007.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     CDS             1645..2895
                     /label=BsDyP coding region
     misc_feature    1645..1647
                     /label=ATG start codon
     misc_feature    2893..2895
                     /label=TAA stop codon
     CDS             1645..2895
                     /label=BBa_K1336003
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatgagc gatgaacaga aaaagccaga acaaattcac
     1681 agacgggaca ttttaaaatg gggagcgatg gcgggggcag ccgttgcgat cggtgccagc
     1741 ggtctcggcg gtctcgctcc gcttgttcag actgcggcta agccatcgaa aaaggatgaa
     1801 aaagaggagg agcagattgt tccgttttac ggaaagcatc aggccggaat cacaactgcc
     1861 catcagacgt atgtctattt tgctgcactg gatgtaacag caaaagacaa aagcgacatc
     1921 attacgttat ttagaaactg gacaagtctc acacagatgc tgacgtctgg caagaaaatg
     1981 tcagcggagc agagaaatca gtatctgccg ccgcaggata caggtgagtc tgctgattta
     2041 tccccttcca acttaacggt cacattcgga ttcgggcctg gcttttttga aaaagacgga
     2101 aaggaccgct tcggactgaa aagcaaaaaa ccgaagcacc tagccgctct tccagcgatg
     2161 cccaatgaca acctggatga aaagcaggga ggcggggaca tctgcattca agtgtgtgca
     2221 gacgacgagc aagtggcgtt tcacgcgctg cgtaacctgc taaatcaagc ggtcggcact
     2281 tgtgaggtcc gttttgtgaa caaaggcttt ttaagcggtg gaaaaaatgg tgaaacaccg
     2341 cgcaacctct tcgggtttaa agatggaaca ggcaaccaga gcacgaagga tgacacgttg
     2401 atgaactcga tcgtgtggat tcagtctggt gagcctgact ggatgacggg cggcacctat
     2461 atggcttttc ggaaaatcaa aatgttcctt gaggtatggg accgctcttc cctcaaggac
     2521 caagaggata ccttcggccg cagaaaaagc tcgggggcgc cttttgggca gaaaaaggaa
     2581 acagatcccg tgaagctgaa tcaaattccg tctaattcac acgtcagtct agcgaaatct
     2641 actgggaaac aaattttgcg aagagctttc tcttacacag aaggacttga tccgaaaacc
     2701 ggctatatgg atgcaggtct cctgtttatc agttttcaaa aaaatcccga caatcagttt
     2761 atcccgatgc tgaaggctct ttcagcaaag gatgcgttaa acgaatatac acaaacaatc
     2821 ggttctgctt tatacgcatg cccaggcggc tgcaaaaaag gagaatatat tgcccagcgt
     2881 ttgctggaat cataa
//