LOCUS       BBa_K1339003            2407 bp    DNA     linear       14-Nov-2024
DEFINITION  RBS-otsA-RBS-otsB-dT
ACCESSION   BBa_K1339003
VERSION     BBa_K1339003.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1443
                     /label=BBa_K1339000
     misc_feature    1456..1459
                     /label=conserved
     RBS             1452..1463
                     /label=BBa_B0034
     CDS             1470..2270
                     /label=BBa_K1339001
     stem_loop       2290..2333
                     /label=stem_loop
     misc_feature    2279..2358
                     /label=BBa_B0010
     terminator      2279..2358
                     /label=BBa_B0010
     stem_loop       2374..2393
                     /label=T7 TE
     misc_feature    2400..2400
                     /label=stop
     polyA_site      2394..2407
                     /label=polya
     misc_feature    2367..2407
                     /label=BBa_B0012
     terminator      2367..2407
                     /label=BBa_B0012
     terminator      2279..2407
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagtcgttta gtcgtagtat ctaaccggat tgcaccacca
       61 gacgagcacg ccgccagtgc cggtggcctt gccgttggca tactgggggc actgaaagcc
      121 gcaggcggac tgtggtttgg ctggagtggt gaaacaggga atgaggatca gccgctaaaa
      181 aaggtgaaaa aaggtaacat tacgtgggcc tcttttaacc tcagcgaaca ggaccttgac
      241 gaatactaca accaattctc caatgccgtt ctctggcccg cttttcatta tcggctcgat
      301 ctggtgcaat ttcagcgtcc tgcctgggac ggctatctac gcgtaaatgc gttgctggca
      361 gataaattac tgccgctgtt gcaagacgat gacattatct ggatccacga ttatcacctg
      421 ttgccatttg cgcatgaatt acgcaaacgg ggagtgaata atcgcattgg tttctttctg
      481 catattcctt tcccgacacc ggaaatcttc aacgcgctgc cgacatatga caccttgctt
      541 gaacagcttt gtgattatga tttgctgggt ttccagacag aaaacgatcg tctggcgttc
      601 ctggattgtc tttctaacct gacccgcgtc acgacacgta gcgcaaaaag ccatacagcc
      661 tggggcaaag catttcgaac agaagtctac ccgatcggca ttgaaccgaa agaaatagcc
      721 aaacaggctg ccgggccact gccgccaaaa ctggcgcaac ttaaagcgga actgaaaaac
      781 gtacaaaata tcttttctgt cgaacggctg gattattcca aaggtttgcc agagcgtttt
      841 ctcgcctatg aagcgttgct ggaaaaatat ccgcagcatc atggtaaaat tcgttatacc
      901 cagattgcac caacgtcgcg tggtgatgtg caagcctatc aggatattcg tcatcagctc
      961 gaaaatgaag ctggacgaat taatggtaaa tacgggcaat taggctggac gccgctttat
     1021 tatttgaatc agcattttga ccgtaaatta ctgatgaaaa tattccgcta ctctgacgtg
     1081 ggcttagtga cgccactgcg tgacgggatg aacctggtag caaaagagta tgttgctgct
     1141 caggacccag ccaatccggg cgttcttgtt ctttcgcaat ttgcgggagc ggcaaacgag
     1201 ttaacgtcgg cgttaattgt taacccctac gatcgtgacg aagttgcagc tgcgctggat
     1261 cgtgcattga ctatgtcgct ggcggaacgt atttcccgtc atgcagaaat gctggacgtt
     1321 atcgtgaaaa acgatattaa ccactggcag gagtgcttca ttagcgacct aaagcagata
     1381 gttccgcgaa gcgcggaaag ccagcagcgc gataaagttg ctacctttcc aaagcttgcg
     1441 tagtactaga gaaagaggag aaatactaga tgacagaacc gttaaccgaa acccctgaac
     1501 tatccgcgaa atatgcctgg ttttttgatc ttgatggaac gctggcggaa atcaaaccgc
     1561 atcccgatca ggtcgtcgtg cctgacaata ttctgcaagg actacagcta ctggcaaccg
     1621 caagtgatgg tgcattggca ttgatatcag ggcgctcaat ggtggagctt gacgcactgg
     1681 caaaacctta tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg
     1741 gtaaaacaca tatcgttcat ctgccggatg cgattgcgcg tgatattagc gtgcaactgc
     1801 atacagtcat cgctcagtat cccggcgcgg agctggaggc gaaagggatg gcttttgcgc
     1861 tgcattatcg tcaggctccg cagcatgaag acgcattaat gacattagcg caacgtatta
     1921 ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag atcaaaccga
     1981 gaggtaccag taaaggtgag gcaattgcag cttttatgca ggaagctccc tttatcgggc
     2041 gaacgcccgt atttctgggc gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc
     2101 gactgggcgg aatgtcagta aaaattggca caggtgcaac tcaggcatca tggcgactgg
     2161 cgggtgtgcc ggatgtctgg agctggcttg aaatgataac caccgcatta caacaaaaaa
     2221 gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa tactagagcc
     2281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2341 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2401 gtttata
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