LOCUS BBa_K1339013 3625 bp DNA linear 22-Nov-2024 DEFINITION Pbad-RBS-otsB-RBS-otsA-dT ACCESSION BBa_K1339013 VERSION BBa_K1339013.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2037 /label=BBa_K1339001 misc_feature 2050..2053 /label=conserved RBS 2046..2057 /label=BBa_B0034 CDS 2064..3488 /label=BBa_K1339000 stem_loop 3508..3551 /label=stem_loop misc_feature 3497..3576 /label=BBa_B0010 terminator 3497..3576 /label=BBa_B0010 stem_loop 3592..3611 /label=T7 TE misc_feature 3618..3618 /label=stop polyA_site 3612..3625 /label=polya misc_feature 3585..3625 /label=BBa_B0012 terminator 3585..3625 /label=BBa_B0012 terminator 3497..3625 /label=BBa_B0015 misc_feature 1219..3625 /label=BBa_K1339004 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga cagaaccgtt aaccgaaacc 1261 cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc 1321 aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg 1381 gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac 1441 gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac 1501 atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg 1561 caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct 1621 tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa 1681 cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc 1741 aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt 1801 atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc 1861 gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg 1921 cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa 1981 caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaatac 2041 tagagaaaga ggagaaatac tagatgagtc gtttagtcgt agtatctaac cggattgcac 2101 caccagacga gcacgccgcc agtgccggtg gccttgccgt tggcatactg ggggcactga 2161 aagccgcagg cggactgtgg tttggctgga gtggtgaaac agggaatgag gatcagccgc 2221 taaaaaaggt gaaaaaaggt aacattacgt gggcctcttt taacctcagc gaacaggacc 2281 ttgacgaata ctacaaccaa ttctccaatg ccgttctctg gcccgctttt cattatcggc 2341 tcgatctggt gcaatttcag cgtcctgcct gggacggcta tctacgcgta aatgcgttgc 2401 tggcagataa attactgccg ctgttgcaag acgatgacat tatctggatc cacgattatc 2461 acctgttgcc atttgcgcat gaattacgca aacggggagt gaataatcgc attggtttct 2521 ttctgcatat tcctttcccg acaccggaaa tcttcaacgc gctgccgaca tatgacacct 2581 tgcttgaaca gctttgtgat tatgatttgc tgggtttcca gacagaaaac gatcgtctgg 2641 cgttcctgga ttgtctttct aacctgaccc gcgtcacgac acgtagcgca aaaagccata 2701 cagcctgggg caaagcattt cgaacagaag tctacccgat cggcattgaa ccgaaagaaa 2761 tagccaaaca ggctgccggg ccactgccgc caaaactggc gcaacttaaa gcggaactga 2821 aaaacgtaca aaatatcttt tctgtcgaac ggctggatta ttccaaaggt ttgccagagc 2881 gttttctcgc ctatgaagcg ttgctggaaa aatatccgca gcatcatggt aaaattcgtt 2941 atacccagat tgcaccaacg tcgcgtggtg atgtgcaagc ctatcaggat attcgtcatc 3001 agctcgaaaa tgaagctgga cgaattaatg gtaaatacgg gcaattaggc tggacgccgc 3061 tttattattt gaatcagcat tttgaccgta aattactgat gaaaatattc cgctactctg 3121 acgtgggctt agtgacgcca ctgcgtgacg ggatgaacct ggtagcaaaa gagtatgttg 3181 ctgctcagga cccagccaat ccgggcgttc ttgttctttc gcaatttgcg ggagcggcaa 3241 acgagttaac gtcggcgtta attgttaacc cctacgatcg tgacgaagtt gcagctgcgc 3301 tggatcgtgc attgactatg tcgctggcgg aacgtatttc ccgtcatgca gaaatgctgg 3361 acgttatcgt gaaaaacgat attaaccact ggcaggagtg cttcattagc gacctaaagc 3421 agatagttcc gcgaagcgcg gaaagccagc agcgcgataa agttgctacc tttccaaagc 3481 ttgcgtagta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3541 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3601 tcgggtgggc ctttctgcgt ttata //