LOCUS       BBa_K1341007             981 bp    DNA     linear       23-Dec-2024
DEFINITION  Afl   Ⅲ site&LTTGFP&AFL III SITE
ACCESSION   BBa_K1341007
VERSION     BBa_K1341007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..10
                     /label=BBa_K1341005
     misc_feature    19..38
                     /label=LACO1
     misc_feature    39..43
                     /label=-35
     misc_feature    45..61
                     /label=tetRO1
     misc_feature    62..67
                     /label=-10
     misc_feature    68..87
                     /label=tetRO2
     RBS             88..100
                     /label=specialized  RBS
     promoter        19..100
                     /label=BBa_K1341022
     CDS             107..826
                     /label=GFP protein
     CDS             107..826
                     /label=BBa_E0040
     stem_loop       846..889
                     /label=stem_loop
     misc_feature    835..914
                     /label=BBa_B0010
     terminator      835..914
                     /label=BBa_B0010
     stem_loop       930..949
                     /label=T7 TE
     misc_feature    956..956
                     /label=stop
     polyA_site      950..963
                     /label=polya
     misc_feature    923..963
                     /label=BBa_B0012
     terminator      923..963
                     /label=BBa_B0012
     misc_feature    19..963
                     /label=BBa_K1341000
     misc_feature    972..981
                     /label=BBa_K1341005
ORIGIN
        1 ccacatgtgg tactagagaa attgtgagcg gataacaatt gacatcccta tcagtgatag
       61 agatactatc cctatcagtg atagagaagg aaaccggttc tactagatgc gtaaaggaga
      121 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      181 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      241 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      301 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      361 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      421 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      481 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      541 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      601 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      661 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      721 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      781 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 atatactaga gccacatgtg g
//