LOCUS       BBa_K1341010            1004 bp    DNA     linear       22-Dec-2024
DEFINITION  OUTPUT SYS(GFP)LOGIC GATE IN Graph Theory 2
ACCESSION   BBa_K1341010
VERSION     BBa_K1341010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LACO1
     misc_feature    21..25
                     /label=-35
     misc_feature    27..43
                     /label=tetRO1
     misc_feature    44..49
                     /label=-10
     misc_feature    50..69
                     /label=tetRO2
     RBS             70..82
                     /label=specialized  RBS
     promoter        1..69
                     /label=BBa_K1341022
     RBS             127..141
                     /label=B0030
     misc_feature    78..83
                     /label=Sal I
     misc_feature    92..97
                     /label=Bam HI
     misc_feature    103..108
                     /label=Bgl II
     misc_feature    118..123
                     /label=Mlu I
     RBS             78..141
                     /label=BBa_K121016
     CDS             148..867
                     /label=GFP protein
     CDS             148..867
                     /label=BBa_E0040
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     misc_feature    78..1004
                     /label=BBa_K1341009
ORIGIN
        1 aaattgtgag cggataacaa ttgacatccc tatcagtgat agagatacta tccctatcag
       61 tgatagagat actagaggtc gactgacgac tggatcctgt cggagatctg tcggataacg
      121 cgtgagatta aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
      181 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      241 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      301 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      361 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      421 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      481 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      541 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      601 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      661 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      721 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      781 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      841 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//