LOCUS       BBa_K1343002             337 bp    DNA     linear       27-Dec-2024
DEFINITION  plac promoter with double terminator
ACCESSION   BBa_K1343002
VERSION     BBa_K1343002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     stem_loop       220..263
                     /label=stem_loop
     misc_feature    209..288
                     /label=BBa_B0010
     terminator      209..288
                     /label=BBa_B0010
     stem_loop       304..323
                     /label=T7 TE
     misc_feature    330..330
                     /label=stop
     polyA_site      324..337
                     /label=polya
     misc_feature    297..337
                     /label=BBa_B0012
     terminator      297..337
                     /label=BBa_B0012
     terminator      209..337
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      301 actggctcac cttcgggtgg gcctttctgc gtttata
//