##gff-version 3
##sequence-region BBa_K1343003 1 1147
BBa_K1343003	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1343003	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1343003	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1343003	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1343003	.	terminator	1019	1147	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1343003	.	terminator	1107	1147	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1343003	.	polyA_site	1134	1147	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1343003	.	stem_loop	1114	1133	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1343003	.	engineered_region	1107	1147	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1343003	.	stop_codon	1140	1140	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1343003	.	terminator	1019	1098	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1343003	.	stem_loop	1030	1073	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1343003	.	engineered_region	1019	1098	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1343003	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1343003	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1343003	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1343003	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1343003	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1343003	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1343003	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1343003	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1343003	.	CDS	230	985	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1343003	.	CDS	230	979	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1343003	.	engineered_region	230	985	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1343003	.	point_mutation	403	403	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1343003	.	engineered_region	230	231	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1343003	.	engineered_tag	986	1010	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1343003	.	point_mutation	721	721	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_K1343003
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaaaaa
cataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctta
tctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgata
tttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagt
agattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaat
gtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaa
tgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaat
tgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaa
aaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactg
tcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaac
aggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata