LOCUS       BBa_K1343004            3801 bp    DNA     linear       23-Dec-2024
DEFINITION  Plac-CI-T7
ACCESSION   BBa_K1343004
VERSION     BBa_K1343004.1  
FEATURES             Location/Qualifiers
     misc_feature    135..156
                     /label=Barcode Label 1
     promoter        1..112
                     /label=Plac
     misc_feature    157..178
                     /label=Barcode Label 4
     misc_feature    113..134
                     /label=Barcode Label 6
     RBS             179..193
                     /label=BBa_B0030: Strong RBS
     CDS             200..913
                     /label=CI lambda repressor
     misc_feature    914..935
                     /label=Barcode Label 3
     RBS             936..950
                     /label=BBa_B0030: Strong RBS
     misc_feature    3755..3801
                     /label=T7 terminator
     misc_feature    3653..3674
                     /label=Barcode Label 5
     misc_feature    3631..3652
                     /label=Barcode Label 2
     misc_feature    3675..3754
                     /label=BBa_B0010:T1 (terminator) from E. coli
     misc_feature    3609..3630
                     /label=Barcode Label 4
     CDS             957..3608
                     /label=BBa_K145001:T7 RNA polymerase (BL21DE3)
ORIGIN
        1 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
       61 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca cacctcgagg
      121 agatctgttt aaacccaatt gctaaattca acctccccga ttcctgttaa tttgaaggat
      181 taaagaggag aaaccaccaa tgagcacaaa aaagaaacca ttaacacaag agcagcttga
      241 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca
      301 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg
      361 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt
      421 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat
      481 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat
      541 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac
      601 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc
      661 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc
      721 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa
      781 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat
      841 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga
      901 gacgtttggc taaccaatat ccttagctga tcaccattaa agaggagaaa ccaccaatga
      961 acacgattaa catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca
     1021 acactctggc tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg
     1081 agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg
     1141 gtgaggttgc ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga
     1201 ttgcacgcat caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag
     1261 ccttccagtt cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca
     1321 ctctggcttg cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg
     1381 gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact
     1441 tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat
     1501 ttatgcaagt tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt
     1561 cttcgtggca taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg
     1621 agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg
     1681 agactatcga actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg
     1741 ctggcatctc tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta
     1801 ctggtggtgg ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta
     1861 agaaagcact gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca
     1921 ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca
     1981 ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga
     2041 tgaaaccgga agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg
     2101 ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg tatcagcctt gagttcatgc
     2161 ttgagcaagc caataagttt gctaaccata aggccatctg gttcccttac aacatggact
     2221 ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag
     2281 gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa
     2341 tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca
     2401 ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt
     2461 gggctgagca agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac
     2521 agcaccacgg cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg
     2581 gcatccagca cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc
     2641 ttcctagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc
     2701 tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca
     2761 ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg
     2821 cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca
     2881 aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg
     2941 gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt
     3001 gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg
     3061 ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc
     3121 gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc
     3181 ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta
     3241 acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact
     3301 ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt
     3361 acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg
     3421 cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac
     3481 tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc
     3541 cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt
     3601 tcgcgtaacc gattcctgtt aatttgaagg gaatccttaa ttgatcacac ggccacagtt
     3661 gacattagat atggccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3721 tcgttttatc tgttgtttgt cggtgaacgc tctctagcat aaccccttgg ggcctctaaa
     3781 cgggtcttga ggggtttttt g
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