LOCUS BBa_K1343007 1048 bp DNA linear 23-Dec-2024 DEFINITION Ptet-CI ACCESSION BBa_K1343007 VERSION BBa_K1343007.1 FEATURES Location/Qualifiers promoter 1..54 /label=BBa_R0040:TetR repressible promoter misc_feature 55..76 /label=barcode 6 misc_feature 77..98 /label=barcode 1 misc_feature 99..120 /label=barcode 4 RBS 121..135 /label=BBa_B0030: Strong RBS CDS 142..855 /label=CI lambda repressor misc_feature 856..877 /label=barcode 3 misc_feature 878..899 /label=barcode 2 misc_feature 900..921 /label=barcode 5 misc_feature 922..1001 /label=BBa_B0010:T1 (terminator) from E. coli misc_feature 1002..1048 /label=T7 terminator ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcaccctcga 61 ggagatctgt ttaaacccaa ttgctaaatt caacctcccc gattcctgtt aatttgaagg 121 attaaagagg agaaaccacc aatgagcaca aaaaagaaac cattaacaca agagcagctt 181 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 241 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 301 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 361 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 421 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 481 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 541 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 601 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 661 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 721 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 781 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 841 gagacgtttg gctaaccaat atccttagct gatcaccgaa tccttaattg atcacacggc 901 cacagttgac attagatatg gccaggcatc aaataaaacg aaaggctcag tcgaaagact 961 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctagcataac cccttggggc 1021 ctctaaacgg gtcttgaggg gttttttg //