LOCUS       BBa_K1343008            1427 bp    DNA     linear       05-Nov-2024
DEFINITION  Ptet-LacI
ACCESSION   BBa_K1343008
VERSION     BBa_K1343008.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=BBa_R0040:TetR repressible promoter
     misc_feature    55..76
                     /label=barcode 6
     misc_feature    77..98
                     /label=barcode 1
     misc_feature    99..120
                     /label=barcode 4
     RBS             121..135
                     /label=BBa_B0030: Strong RBS
     CDS             142..1233
                     /label=lacI
     misc_feature    1234..1255
                     /label=barcode 3
     misc_feature    1256..1277
                     /label=barcode 2
     misc_feature    1278..1299
                     /label=barcode 5
     misc_feature    1300..1379
                     /label=BBa_B0010:T1 (terminator) from E. coli
     misc_feature    1380..1427
                     /label=T7 terminator
ORIGIN
        1 gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg agcaccctcg
       61 aggagatctg tttaaaccca attgctaaat tcaacctccc cgattcctgt taatttgaag
      121 gattaaagag gagaaaccac caatggtgaa tgtgaaacca gtaacgttat acgatgtcgc
      181 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
      241 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
      301 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
      361 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
      421 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
      481 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
      541 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
      601 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
      661 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
      721 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
      781 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
      841 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
      901 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
      961 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     1021 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     1081 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     1141 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1201 acgacaggtt tcccgactgg aaagcgggca gtaaccaata tccttagctg atcaccgaat
     1261 ccttaattga tcacacggcc acagttgaca ttagatatgg ccaggcatca aataaaacga
     1321 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1381 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttg
//