BBa_K1343016 1 BBa_K1343016 Pcat->RBS->TaZ->dTer 2014-10-04T11:00:00Z 2015-05-08T01:09:59Z Pcat: BBa_I14033 RBS: BBa_B0034 TaZ: BBa_C0082 dTer: BBa_B0015 This part generates the TaZ receptor. The TaZ is under a Pcat constitutive promoter. TaZ is a fused Tar-EnvZ receptor meant for Aspartate dependent response, by activating the EnvZ/OmpR two component signalling system and thus activating the PompC promoter. We used this part for prove-of-concept for low concentration detection. By using this TaZ Generator we were able to test different concentrations of Aspartate and their effect on the PompC promoter present in another plasmid. false false _1718_ 0 20669 9 It's complicated true We had to add a gggggg sequance between RBS and TaZ sequances. false Karen Joseph annotation2394318 1 dTer range2394318 1 1765 1893 annotation2394317 1 Taz range2394317 1 57 1572 annotation2394315 1 Pcat range2394315 1 1 38 annotation2394316 1 RBS range2394316 1 39 50 BBa_K1343016_sequence 1 ggcacgtaagaggttccaactttcaccataatgaaacaaaagaggagaaaggggggatgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgatttcaccataatgaaacaaaagaggagaaaggggggccaggcatcaaataaaacgaaaggctcagtcgaaagactgcaccataatgaaacaaaagaggagaaaggggggccaggcatcaaataaaacgaaagaatgaaacaaaagaggagaaaggggggccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z