BBa_K592008
1
PT5lac
T5-lac Promoter
2011-09-10T11:00:00Z
2015-05-08T01:12:48Z
Phage T5
Released HQ 2013
T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.
false
false
_763_
0
7929
9
In stock
false
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
false
Erik Lundin
annotation2135661
1
-10 signal
range2135661
1
87
93
annotation2127135
1
T5-lac promoter
range2127135
1
1
126
annotation2135656
1
lacO1 site
range2135656
1
1
26
annotation2135657
1
lacO1 site
range2135657
1
69
86
annotation2135658
1
lacOi site
range2135658
1
94
123
annotation2135659
1
-35 signal
range2135659
1
63
68
BBa_K1344000
1
1018
1018 synthetic cationic peptide
2014-10-03T11:00:00Z
2015-05-08T01:10:00Z
This part is synthetized.
Peptide 1018 is a synthetic cationic peptides, derived from natural peptides such as the human cathelicidin LL-37 and the bovine peptide indolicidin. This part work as biofilm inhibitory compounds. Anti-biofilm peptides are similar to cationic antimicrobial peptides (which are active against planktonic bacteria), comprising both cationic and hydrophobic amino acids.
false
false
_1719_
0
20150
9
In stock
true
This part will have broad spectrum effect on gram-negative and gram-positive bacterias
false
UI Indonesia 2014
annotation2393869
1
1018
range2393869
1
1
45
annotation2393870
1
Stop
range2393870
1
40
45
annotation2393871
1
ATG
range2393871
1
1
1
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1344001
1
BBa_K1344001
T5-lac-1018
2014-10-04T11:00:00Z
2015-05-08T01:10:00Z
1018 is synthetized, and the promoter + rbs was taken from the registry, BBa_K592008 and BBa_B0034
This part will expressing 1018 by inducing IPTG at various concentration
false
false
_1719_
0
20150
9
It's complicated
true
This inhibitory and killing peptide will works only when induced with IPTG
false
UI Indonesia 2014
component2400459
1
BBa_K592008
component2400464
1
BBa_K1344000
component2400461
1
BBa_B0034
annotation2400459
1
BBa_K592008
range2400459
1
1
126
annotation2400461
1
BBa_B0034
range2400461
1
135
146
annotation2400464
1
BBa_K1344000
range2400464
1
153
197
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1344000_sequence
1
atgcgtcgttggattcgtgttgctgttattctgcgcgtgtaataa
BBa_K1344001_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagaaagaggagaaatactagatgcgtcgttggattcgtgttgctgttattctgcgcgtgtaataa
BBa_K592008_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z