LOCUS BBa_K1344008 5285 bp DNA linear 22-Nov-2024 DEFINITION TolC-HlyB-HlyD Canal for Hemolysin-tag Secretion ACCESSION BBa_K1344008 VERSION BBa_K1344008.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS misc_feature 1..1539 /label=BBa_K554009 CDS 16..1533 /label=TolC misc_feature 1534..1539 /label=stop misc_feature 1548..5285 /label=BBa_K1166002 RBS 1554..1565 /label=BBa_B0034 misc_feature 1566..1571 /label=spacer CDS 1572..3692 /label=HlyB misc_feature 3693..3698 /label=stop RBS 3699..3710 /label=BBa_B0034 misc_feature 3711..3716 /label=spacer CDS 3717..5150 /label=HlyD misc_feature 5151..5156 /label=stop stem_loop 5157..5285 /label=BBa_B0015 ORIGIN 1 attaaagagg agaaaatgat tgccctgaac actgcctcgc cacaggggat gcagatgaag 61 aagctcctgc cgatcctgat cgggctaagt ctttccgggt tcagctcact ttcacaagcc 121 gagaatctta tgcaagtata tcagcaggca cggctgtcga atccagagct tcgtaaatca 181 gcggccgatc gagatgctgc gttcgagaaa attaatgagg cccggtcacc gctgttacca 241 cagctgggtc tcggcgcgga ttatacatac agcaacggct accgtgatgc gaatggaata 301 aatagtaacg caaccagcgc aagcttacaa ctgacacagt cgatcttcga tatgtcgaaa 361 tggcgcgcac taacgcttca ggaaaaagca gccgggatcc aagatgtaac ttatcagacc 421 gaccagcaaa cattgatttt gaatactgca accgcttact ttaacgttct gaatgccatc 481 gacgtgctga gctacaccca ggcacaaaag gaagccattt atcgtcaact tgaccagact 541 acgcagcggt ttaacgttgg gttagtagca atcaccgatg ttcaaaatgc gcgtgcgcag 601 tatgataccg ttctggccaa tgaggtcacc gcacgcaaca atcttgataa tgcggttgaa 661 cagttacgtc agattaccgg gaactattat cccgagctgg cagcgctgaa tgtggaaaat 721 tttaaaactg acaaacctca acctgtcaat accctgctca aggaagccga gaaacgcaac 781 ctgtctctat tgcaagctcg cctgtctcaa gacctggctc gggagcaaat acgccaggca 841 caggacggac atctgcccac actggacctg accgcaagta ctgggatatc cgacaccagt 901 tactctggat caaaaactcg tggcgccgcc ggaacacaat acgatgactc aaatatgggc 961 cagaacaagg ttggcctgtc cttcagttta ccgatctacc agggcgggat ggtgaatagt 1021 caagtaaaac aggcgcagta taactttgtc ggtgcatcag agcaactgga atcagctcac 1081 cgtagcgtcg tacagactgt ccggagtagc tttaacaaca ttaacgcgag tatctcatca 1141 atcaatgctt acaagcaagc agtggtttct gcgcagtctt ctttggatgc catggaagca 1201 ggctattcag tgggtactcg cacgattgtt gatgttctcg acgctaccac aactctctac 1261 aacgcgaaac aggaattagc gaatgcccgt tataactact taattaacca actgaatatc 1321 aaatctgccc taggtactct gaatgagcaa gacttgcttg cgttaaataa cgccctgtcc 1381 aaacctgtct caaccaatcc tgagaacgta gcacctcaga cccctgaaca gaacgccatt 1441 gcggacggtt acgcccctga cagtcctgcg cctgtggtcc agcagacgtc agcgcgcact 1501 accacttcaa acgggcataa tccgttccgc aattaataat actagcggct agcaaagagg 1561 agaaatacta gatggactcc tgtcacaaga tcgactacgg cctgtatgca ctggaaatcc 1621 tggctcaata ccacaacgtg tcggtcaacc cggaagaaat taaacaccgc tttgacacgg 1681 atggtaccgg cctgggtctg acgtcgtggc tgctggccgc aaagagcctg gaactgaaag 1741 tgaaacaggt gaagaagacg attgaccgcc tgaactttat cagcctgcca gctctggttt 1801 ggcgcgaaga tggtcgtcat ttcatcctga cgaaagtgtc gaaggaagca aatcgctatc 1861 tgatcttcga cctggaacag cgcaatccgc gtgtgctgga acaaagcgaa tttgaagcgc 1921 tgtaccaagg tcacatcatt ctgattgcct cacgctccag cgttacgggc aaactggcca 1981 agttcgattt tacctggttt attccggcca tcattaaata tcgtaagatt ttcatcgaaa 2041 ccctggtcgt gagcgtgttt ctgcaactgt tcgcactgat tacgccgctg tttttccagg 2101 tggtcatgga taaggtgctg gtccatcgtg gtttttcgac cctgaacgtg atcacggttg 2161 ctctgtcagt tgtggtggtg tttgaaatta ttctgagcgg tctgcgtacc tacatctttg 2221 cacactcgac ctctcgtatc gacgttgaac tgggtgcgaa gctgttccgc cacctgctgg 2281 cactgccgat tagctacttt gaaagccgtc gtgttggtga taccgtggcc cgcgtgcgtg 2341 aactggacca aattcgtaat tttctgaccg gccaggccct gacgtccgtc ctggatctgc 2401 tgttctcgtt tattttcttt gccgttatgt ggtattacag tccgaagctg acgctggtga 2461 ttctgtttag tctgccgtgc tatgcggcat ggagcgtttt tatcagcccg attctgcgtc 2521 gtcgcctgga tgacaaattt agccgtaatg ccgacaacca gtcatttctg gtcgaatccg 2581 ttaccgcaat taataccatc aaggctatgg cagtttcacc gcaaatgacc aacatttggg 2641 ataaacaact ggcaggttac gttgcagcag gtttcaaggt gaccgttctg gctaccattg 2701 gccagcaggg tattcaactg atccagaaaa cggtgatgat catcaacctg tggctgggtg 2761 ctcatctggt gatttctggc gatctgagca ttggtcaact gattgccttc aatatgctgg 2821 cgggccagat cgtggccccg gtgatccgtc tggcacagat ttggcaagac ttccaacagg 2881 tcggtattag tgtgacgcgt ctgggcgacg tgctgaacag cccgaccgaa tcctatcatg 2941 gcaaactggc tctgccggaa atcaatggta acattacgtt ccgcaatatt cgtttccgct 3001 acaaaccgga ttcgccggtg attctggata acatcaacct gtctatcaaa cagggtgaag 3061 tgattggtat tgtgggtcgc tccggttcag gtaagagtac gctgaccaaa ctgatccagc 3121 gtttttatat cccggaaaac ggtcaagtcc tgattgacgg ccatgatctg gcactggcgg 3181 acccgaactg gctgcgccgt caagtgggtg tggtgctgca agacaatgtg ctgctgaacc 3241 gcagcattat tgacaatatc agtctggcaa atccgggcat gtcggtggaa aaggtcatct 3301 acgctgccaa gctggcaggc gcccacgatt tcattagcga actgcgcgaa ggctataaca 3361 cgattgtggg cgaacagggt gcaggcctgt ctggcggtca acgtcagcgc attgcaattg 3421 ctcgcgcgct ggtcaacaat ccgaaaattc tgatttttga cgaagcgacc agtgccctgg 3481 attatgaaag cgaacatatt atcatgcgta acatgcacaa aatctgcaag ggccgcacgg 3541 tcattatcat tgcgcaccgt ctgagtaccg tcaagaacgc ggatcgtatc attgtgatgg 3601 aaaagggtaa aatcgtggaa caaggcaagc ataaggaact gctgtcggaa ccggaaagcc 3661 tgtactcgta tctgtatcaa ctgcaatcgg attaataaaa agaggagaaa tactagatga 3721 agacgtggct gatgggcttt agtgaatttc tgctgcgtta caaactggtg tggtcggaaa 3781 cctggaagat tcgcaaacaa ctggataccc cggtgcgcga aaaagacgaa aacgaatttc 3841 tgccagcgca tctggaactg attgaaacgc cggtcagtcg tcgcccgcgt ctggtggcgt 3901 attttatcat gggcttcctg gttattgccg tcatcctgtc cgttctgggt caggtggaaa 3961 ttgttgcaac cgctaacggc aaactgacgc tgagcggtcg ctctaaagaa attaagccga 4021 tcgaaaatag catcgtcaaa gaaattatcg tgaaggaagg cgaaagcgtg cgtaaaggtg 4081 atgtcctgct gaagctgacc gccctgggcg cagaagcaga caccctgaaa acgcagagct 4141 ctctgctgca aacccgtctg gaacagacgc gctatcaaat tctgtcacgc tcgatcgaac 4201 tgaacaaact gccggaactg aagctgccgg atgaaccgta ctttcagaat gtgagcgaag 4261 aagaagtgct gcgtctgacc agcctgatta aagaacagtt ctctacgtgg cagaaccaaa 4321 agtaccaaaa ggaactgaat ctggataaaa agcgtgcaga acgcctgacc attctggctc 4381 gtatcaaccg ctacgaaaat ctgtcacgtg tggaaaaatc gcgcctggat gactttcgta 4441 gtctgctgca taaacaggca attgctaagc acgcagttct ggaacaagaa aacaaatatg 4501 tcgaagcggc caatgaactg cgcgtgtaca agagccaact ggaacaaatt gaaagtgaaa 4561 tcctgtccgc caaagaagaa tatcaactgg tgacccaact gttcaaaaat gaaattctgg 4621 ataagctgcg tcagaccacg gacaacatcg aactgctgac cctggaactg gaaaagaacg 4681 aagaacgtca gcaagcaagt gtgattcgtg ctccggtttc cggtaaagtc cagcaactga 4741 aggttcatac cgaaggcggt gtggttacca cggcagaaac gctgatggtc atcgtgccgg 4801 aagatgacac cctggaagtg acggctctgg ttcagaacaa agatattggc tttatcaacg 4861 ttggtcagaa tgcgattatc aaggtcgaag ccttcccgta cacccgctat ggctacctgg 4921 tcggtaaagt gaagaacatt aatctggatg cgatcgaaga ccagaaactg ggcctggtgt 4981 tcaatgttat tgtctcagtg gaagaaaacg atctgtcgac cggcaataaa cacattccgc 5041 tgagttccgg tatggcggtt accgccgaaa tcaaaacggg tatgcgttct gtcatctcct 5101 atctgctgtc gccgctggaa gaatctgtca cggaatcgct gcatgaacgt taataaccag 5161 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 5221 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 5281 ttata //