LOCUS       BBa_K1350000             594 bp    DNA     linear       23-Dec-2024
DEFINITION  Ccdb E.coli suicide IPTG
ACCESSION   BBa_K1350000
VERSION     BBa_K1350000.1  
FEATURES             Location/Qualifiers
     promoter        1..126
                     /label=T5-lac promoter
     misc_feature    1..26
                     /label=lacO1 site
     misc_feature    69..86
                     /label=lacO1 site
     misc_feature    94..123
                     /label=lacOi site
     promoter        63..68
                     /label=-35 signal
     promoter        87..93
                     /label=-10 signal
     promoter        1..126
                     /label=BBa_K592008
     RBS             135..145
                     /label=RBS-4\Weaker
     RBS             141..144
                     /label=RBS
     misc_feature    135..145
                     /label=BBa_B0033
     RBS             135..145
                     /label=BBa_B0033
     misc_feature    152..154
                     /label=start
     misc_feature    455..457
                     /label=stop
     CDS             152..454
                     /label=cds
     CDS             152..457
                     /label=BBa_K145151
     stem_loop       477..520
                     /label=stem_loop
     misc_feature    466..545
                     /label=BBa_B0010
     terminator      466..545
                     /label=BBa_B0010
     stem_loop       561..580
                     /label=T7 TE
     misc_feature    587..587
                     /label=stop
     polyA_site      581..594
                     /label=polya
     misc_feature    554..594
                     /label=BBa_B0012
     terminator      554..594
                     /label=BBa_B0012
     terminator      466..594
                     /label=BBa_B0015
     misc_feature    135..594
                     /label=BBa_K581008
ORIGIN
        1 tgtggaattg tgagcggata acaattacga gcttcatgca cagtgaaatc atgaaaaatt
       61 tatttgcttt gtgagcggat aacaattata atatgtggaa ttgtgagcgc tcacaattcc
      121 acaacgtact agagtcacac aggactacta gatgcagttt aaggtttaca cctataaaag
      181 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
      241 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
      301 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
      361 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
      421 caaaaacgcc attaacctga tgttctgggg aatataatac tagagccagg catcaaataa
      481 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      541 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//