##gff-version 3
##sequence-region BBa_K1350000 1 594
BBa_K1350000	.	promoter	1	126	.	+	0	ID=BBa_K592008;Name=BBa_K592008
BBa_K1350000	.	promoter	63	68	.	+	0	ID=annotation2135659;Name=-35 signal;Parent=BBa_K592008
BBa_K1350000	.	non_covalent_binding_site	1	26	.	+	0	ID=annotation2135656;Name=lacO1 site;Parent=BBa_K592008
BBa_K1350000	.	non_covalent_binding_site	69	86	.	+	0	ID=annotation2135657;Name=lacO1 site;Parent=BBa_K592008
BBa_K1350000	.	promoter	87	93	.	+	0	ID=annotation2135661;Name=-10 signal;Parent=BBa_K592008
BBa_K1350000	.	promoter	1	126	.	+	0	ID=annotation2127135;Name=T5-lac promoter;Parent=BBa_K592008
BBa_K1350000	.	non_covalent_binding_site	94	123	.	+	0	ID=annotation2135658;Name=lacOi site;Parent=BBa_K592008
BBa_K1350000	.	engineered_region	135	594	.	+	0	ID=BBa_K581008;Name=BBa_K581008
BBa_K1350000	.	CDS	152	457	.	+	0	ID=BBa_K145151;Name=BBa_K145151;Parent=BBa_K581008
BBa_K1350000	.	CDS	152	454	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K1350000	.	start_codon	152	154	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K1350000	.	stop_codon	455	457	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K1350000	.	ribosome_entry_site	135	145	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K581008
BBa_K1350000	.	ribosome_entry_site	141	144	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1350000	.	ribosome_entry_site	135	145	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1350000	.	engineered_region	135	145	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1350000	.	terminator	466	594	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K581008
BBa_K1350000	.	terminator	466	545	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1350000	.	stem_loop	477	520	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1350000	.	engineered_region	466	545	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1350000	.	terminator	554	594	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1350000	.	engineered_region	554	594	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1350000	.	stop_codon	587	587	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1350000	.	polyA_site	581	594	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1350000	.	stem_loop	561	580	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1350000
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcgga
taacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagtcacacaggactactagatgcagt
ttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgg
gcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcat
atcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctg
atctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata