BBa_K1350000
type: ComponentDefinition
persistent identity: BBa K1350000
display id: BBa_K1350000
version: 1
was generated by: iGEM to SBOL conversion
description: Ccdb E.coli suicide IPTG
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:01Z
mutable provenance: "IPTG bomb" kill the E.coli by coding Ccdb toxin protein. The Ccdb come from BBa_K581008(RBS+ccdB+Termintor),and T5-lac Promoter from BBa_K592008
owned by: james
owned by: myers
mutable description: A system designed to kill E.coli when IPTG added . IPTG can efficiently trigger a large numumber of Ccdb protein .And Ccdb protein a can combine with E.coli ，So it can effectively kill the E.coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1725_
m user id: 0
owner id: 20427
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have been thinking of a way, that way we could eliminate E.coli. Now we Ccdb protein-bound T5-lac and make the controls to suit our needs.
star: false
creator: JieRui Chen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K581008
component: BBa_K592008
sequence annotation: BBa_K592008
sequence annotation: BBa_K581008
sequence: https://synbiohub.org/public/igem/BBa_K1350000_sequence/1

BBa_K581008
type: Component
persistent identity: component2387309
display id: component2387309
version: 1
top level: BBa_K1350000
definition: BBa_K581008
access: public

BBa_K592008
type: Component
persistent identity: component2387294
display id: component2387294
version: 1
top level: BBa_K1350000
definition: PT5lac
access: public

BBa_K592008
type: SequenceAnnotation
persistent identity: annotation2387294
display id: annotation2387294
version: 1
top level: BBa_K1350000
location: https://synbiohub.org/public/igem/BBa_K1350000/annotation2387294/range2387294/1
component: BBa_K592008

https://synbiohub.org/public/igem/BBa_K1350000/annotation2387294/range2387294/1
type: Range
persistent identity: range2387294
display id: range2387294
version: 1
top level: BBa_K1350000
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_K581008
type: SequenceAnnotation
persistent identity: annotation2387309
display id: annotation2387309
version: 1
top level: BBa_K1350000
location: https://synbiohub.org/public/igem/BBa_K1350000/annotation2387309/range2387309/1
component: BBa_K581008

https://synbiohub.org/public/igem/BBa_K1350000/annotation2387309/range2387309/1
type: Range
persistent identity: range2387309
display id: range2387309
version: 1
top level: BBa_K1350000
direction: direction/reverse
start: 135
end: 594
orientation: inline

PT5lac
type: ComponentDefinition
persistent identity: BBa K592008
display id: BBa_K592008
version: 1
was generated by: iGEM to SBOL conversion
description: T5-lac Promoter
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Phage T5
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
star: false
creator: Erik Lundin
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacO1 site
sequence annotation: T5-lac promoter
sequence annotation: -35 signal
sequence annotation: -10 signal
sequence annotation: lacOi site
sequence: https://synbiohub.org/public/igem/BBa_K592008_sequence/1

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2135656
display id: annotation2135656
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135656/range2135656/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K592008/annotation2135656/range2135656/1
type: Range
persistent identity: range2135656
display id: range2135656
version: 1
top level: PT5lac
direction: direction/forward
start: 1
end: 26
orientation: inline

T5-lac promoter
type: SequenceAnnotation
persistent identity: annotation2127135
display id: annotation2127135
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2127135/range2127135/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K592008/annotation2127135/range2127135/1
type: Range
persistent identity: range2127135
display id: range2127135
version: 1
top level: PT5lac
direction: direction/forward
start: 1
end: 126
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2135659
display id: annotation2135659
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135659/range2135659/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K592008/annotation2135659/range2135659/1
type: Range
persistent identity: range2135659
display id: range2135659
version: 1
top level: PT5lac
direction: direction/forward
start: 63
end: 68
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2135657
display id: annotation2135657
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135657/range2135657/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K592008/annotation2135657/range2135657/1
type: Range
persistent identity: range2135657
display id: range2135657
version: 1
top level: PT5lac
direction: direction/forward
start: 69
end: 86
orientation: inline

-10 signal
type: SequenceAnnotation
persistent identity: annotation2135661
display id: annotation2135661
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135661/range2135661/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K592008/annotation2135661/range2135661/1
type: Range
persistent identity: range2135661
display id: range2135661
version: 1
top level: PT5lac
direction: direction/forward
start: 87
end: 93
orientation: inline

lacOi site
type: SequenceAnnotation
persistent identity: annotation2135658
display id: annotation2135658
version: 1
top level: PT5lac
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135658/range2135658/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K592008/annotation2135658/range2135658/1
type: Range
persistent identity: range2135658
display id: range2135658
version: 1
top level: PT5lac
direction: direction/forward
start: 94
end: 123
orientation: inline

BBa_K581008
type: ComponentDefinition
persistent identity: BBa K581008
display id: BBa_K581008
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+ccdB+Termintor
created: 2011-09-25T11:00:00Z
modified: 2015-07-09T12:57:45Z
mutable provenance: Escherichia coli.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the cell death protein coding sequence under the control of a relatively weak RBS. It is replicable in non-ccdB resistant strains.
discontinued: true
dominant: false
experience: experience/Works
group u list: _752_
m user id: 4206
owner id: 8589
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: false
mutable notes: Use a relatively weak RBS so the coding sequence would be replicable in non-ccdB resistant strains under the putative promoter in VF region.
star: false
creator: Deng Linna
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0033
component: BBa_B0015
component: BBa_K145151
sequence annotation: BBa_B0033
sequence annotation: BBa_K145151
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K581008_sequence/1

BBa_B0033
type: Component
persistent identity: component2143052
display id: component2143052
version: 1
top level: BBa_K581008
definition: BBa_B0033
access: public

BBa_B0015
type: Component
persistent identity: component2143064
display id: component2143064
version: 1
top level: BBa_K581008
definition: BBa_B0015
access: public

BBa_K145151
type: Component
persistent identity: component2143057
display id: component2143057
version: 1
top level: BBa_K581008
definition: ccdB
access: public

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2143052
display id: annotation2143052
version: 1
top level: BBa_K581008
location: https://synbiohub.org/public/igem/BBa_K581008/annotation2143052/range2143052/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K581008/annotation2143052/range2143052/1
type: Range
persistent identity: range2143052
display id: range2143052
version: 1
top level: BBa_K581008
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation2143057
display id: annotation2143057
version: 1
top level: BBa_K581008
location: https://synbiohub.org/public/igem/BBa_K581008/annotation2143057/range2143057/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_K581008/annotation2143057/range2143057/1
type: Range
persistent identity: range2143057
display id: range2143057
version: 1
top level: BBa_K581008
direction: direction/reverse
start: 18
end: 323
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2143064
display id: annotation2143064
version: 1
top level: BBa_K581008
location: https://synbiohub.org/public/igem/BBa_K581008/annotation2143064/range2143064/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K581008/annotation2143064/range2143064/1
type: Range
persistent identity: range2143064
display id: range2143064
version: 1
top level: BBa_K581008
direction: direction/reverse
start: 332
end: 460
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
mutable provenance: P1010
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the ccdB (control of cell death) gene.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: just one stop codon in the end
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 303
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
top level: ccdB
direction: direction/forward
start: 304
end: 306
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1350000_sequence/1
type: Sequence
persistent identity: BBa K1350000 sequence
display id: BBa_K1350000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagtcacacaggactactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592008_sequence/1
type: Sequence
persistent identity: BBa K592008 sequence
display id: BBa_K592008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K581008_sequence/1
type: Sequence
persistent identity: BBa K581008 sequence
display id: BBa_K581008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggactactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145151_sequence/1
type: Sequence
persistent identity: BBa K145151 sequence
display id: BBa_K145151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z