BBa_K1350001
1
BBa_K1350001
A kind of secretory protein named kil
2014-09-26T11:00:00Z
2015-05-08T01:10:01Z
ATGAGGAAAAGATTTTTTGTGGGAATATTCGCGATAAACCTCCTTGTTGGATGTCAGGCTAACTATATACCTGATGTTCAGGGAGGGACCATCGCACCATCCTCCTCTTCTAAACTGACGGGGATCGCGGTTCAGTAG
fasta form:
MRKRFFVGIF AINLLVGCQA NYIPDVQGGT IAPSSSSKLT GIAVQ
This is a part.Its sequence length is 136bp and it begins with ATG.It will express the protein, and role in the periplasmic space of e. coli.
false
false
_1725_
0
20403
9
It's complicated
true
This sequence is little short. It might be confused with primer dimers after PCR, so your primer designing should be reasonable.
false
Yongyi Wang
BBa_J23104
1
BBa_J23104
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
isolated from library of promoters
replace later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K608002
1
BBa_K608002
strong Promoter and strong RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
assembly from iGEM parts
Released HQ 2013
you can insert your part behind this part so it will be immediatly expressed in e.coli.
false
false
_780_
0
9115
9
In stock
false
cloning via 3a-assembly
false
Julia M??ller
component2128646
1
BBa_J23104
component2128648
1
BBa_B0034
annotation2128646
1
BBa_J23104
range2128646
1
1
35
annotation2128648
1
BBa_B0034
range2128648
1
44
55
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1350002
1
BBa_K1350002
Promotor+RBS(BBa_K608002) + kil protein(BBa_K1350001)
2014-09-26T11:00:00Z
2015-05-08T01:10:01Z
BBa_K608002
This type is a device combined with a strong promoter and strong RBS.It dose not need to be artificially induced can start downstream gene kil gene transcription and expression.
false
false
_1725_
0
20403
9
It's complicated
false
If you need to use the device, it should be connected to a terminator in its downstream before you connect an exogenous gene.
false
Yongyi Wang
component2387755
1
BBa_K608002
component2387756
1
BBa_K1350001
annotation2387755
1
BBa_K608002
range2387755
1
1
55
annotation2387756
1
BBa_K1350001
range2387756
1
62
199
BBa_K1350002_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgaggaaaagattttttgtgggaatattcgcgataaacctccttgttggatgtcaggctaactatatacctgatgttcagggagggaccatcgcaccatcctcctcttctaaactgacggggatcgcggttcagtag
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K608002_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa
BBa_K1350001_sequence
1
atgaggaaaagattttttgtgggaatattcgcgataaacctccttgttggatgtcaggctaactatatacctgatgttcagggagggaccatcgcaccatcctcctcttctaaactgacggggatcgcggttcagtag
BBa_J23104_sequence
1
ttgacagctagctcagtcctaggtattgtgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z