LOCUS       BBa_K1350010            1431 bp    DNA     linear       16-Feb-2025
DEFINITION  mdfa with terminator
ACCESSION   BBa_K1350010
VERSION     BBa_K1350010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     CDS             62..1294
                     /label=BBa_K1350005
     stem_loop       1314..1357
                     /label=stem_loop
     misc_feature    1303..1382
                     /label=BBa_B0010
     terminator      1303..1382
                     /label=BBa_B0010
     stem_loop       1398..1417
                     /label=T7 TE
     misc_feature    1424..1424
                     /label=stop
     polyA_site      1418..1431
                     /label=polya
     misc_feature    1391..1431
                     /label=BBa_B0012
     terminator      1391..1431
                     /label=BBa_B0012
     terminator      1303..1431
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgcaaaat aaattagctt ccggtgccag gcttggacgt caggcgttac ttttccctct
      121 ctgtctggtg ctttacgaat tttcaaccta tatcggcaac gatatgattc aacccggtat
      181 gttggccgtg gtggaacaat atcaggcggg cattgattgg gttcctactt cgatgaccgc
      241 gtatctggcg ggcgggatgt ttttacaatg gctgctgggg ccgctgtcgg atcgtattgg
      301 tcgccgtccg gtgatgctgg cgggagtggt gtggtttatc gtcacctgtc tggcaatatt
      361 gctggcgcaa aacattgaac aattcaccct gttgcgcttc ttgcagggca taagcctctg
      421 tttcattggc gctgtgggat acgccgcaat tcaggaatcc ttcgaagagg cggtttgtat
      481 caagatcacc gcgctgatgg cgaacgtggc gctgattgct ccgctacttg gtccgctggt
      541 gggcgcggcg tggatccatg tgctgccctg ggaggggatg tttgttttgt ttgccgcatt
      601 ggcagcgatc tcctttttcg gtctgcaacg agccatgcct gaaaccgcca cgcgtatagg
      661 cgagaaactg tcactgaaag aactcggtcg tgactataag ctggtgctga agaacggccg
      721 ctttgtggcg ggggcgctgg cgctgggatt cgttagtctg ccgttgctgg cgtggatcgc
      781 ccagtcgccg attatcatca ttaccggcga gcagttgagc agctatgaat atggcttgct
      841 gcaagtgcct attttcgggg cgttaattgc gggtaacttg ctgttagcgc gtctgacctc
      901 gcgccgcacc gtacgttcgc tgattattat gggcggctgg ccgattatga ttggtctatt
      961 ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga ctgccgggtt
     1021 aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat taaccctgtt
     1081 tgccagcgat atgagtaaag gtacggtttc tgccgcgatg ggaatgctgc aaatgctgat
     1141 ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca acggactgtt
     1201 taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta tctttttaaa
     1261 agataaacag atgggaaatt ctcacgaagg gtaatactag agccaggcat caaataaaac
     1321 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1381 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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