BBa_K1351023
1
BBa_K1351023
upp cassette as counter-selection marker
2014-10-03T11:00:00Z
2015-05-08T01:10:01Z
Derived from pUC19_upp, AG Mascher
Originally from Bacillus subtilis 168 genomic DNA
Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate
Will allow negative selection, Will be Implementet in Modified Plasmid Backbones pSB2E, pSB4S, pSB1C to allow resistance free cloning
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: <br>
{|
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
|-
|suffix with AgeI, SpeI, NotI and PstI:
|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCGC</span><span style="color:blue">CTGCAG</span>
|}
Sites of restriction enzymes generating compatible overhangs have the same color:<br>
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Shine-Dalgarno sequence and stop codons are underlined.
false
false
_1726_
0
20069
9
It's complicated
false
Mutation of Freiburg Standard Restriction Sites within the genomic uppcas was needed
Point Mutation of AgeI restriction site by change of G -> C at position 174
Point Mutation of EcoRI restriction site by change of C -> G at position 477
false
Patrick Reith
annotation2393536
1
upp
range2393536
1
100
729
annotation2393535
1
uppcas
range2393535
1
1
826
BBa_K1351023_sequence
1
ttttttgacgatgttcttgaaactcaatgtctttttttgtagaatcaatagaagtgtgtaattgttgatgggacaataaaaaaggagctgaaacacagtatgggaaaggtttatgtatttgatcatcctttaattcagcacaagctgacatatatacggaatgaaaatacaggtacgaaggattttagagagttagtagatgaagtggctacactcatggcatttgaaattacccgcgatcttcctctggaagaagtggatatcaatacacccgttcaggctgcgaaatcgaaagtcatctcagggaaaaaactcggagtggttcctatcctcagagcaggattgggaatggttgacggcattttaaagctgattcctgcggcaaaagtgggacatgtcggcctttaccgtgatccagaaaccttaaaacccgtggaatactatgtcaagcttccttctgatgtggaagagcgtgagttcatcgtggttgacccgatgctcgctacaggcggttccgcagttgaagccattcacagccttaaaaaacgcggtgcgaaaaatatccgtttcatgtgtcttgtagcagcgccggagggtgtggaagaattgcagaagcatcattcggacgttgatatttacattgcggcgctagatgaaaaattaaatgaaaaaggatatattgttccaggtctcggagatgcgggtgaccgcatgtttggaacaaaataaaaaatgaaatccccaaaagggggtttcatttttttatccagttttttgctattcggtgaatctgtatacaattataggtgaaaatgtgaacattctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z