BBa_K1351028
1
BBa_K1351028
LMU Bacillus RBS collection 1
2014-10-05T11:00:00Z
2015-06-01T08:09:54Z
Synthetic
This part was generated in a modified version of RFC25, and has the following prefix and suffix: <br>{||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color:<br><span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.
false
false
_1726_
4206
20062
9
In stock
false
The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
false
Florian Riemer
BBa_K1351028_sequence
1
aaggagggata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z