BBa_K1351030
1
BBa_K1351030
LMU Bacillus RBS collection 3
2014-10-05T11:00:00Z
2015-06-01T08:10:10Z
Synthetic
"This part was generated in a modified version of RFC25, and has the following prefix and suffix:
{|
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|<span style=""color:blue"">GAATTC</span><span style=""color:green"">GCGGCCGC</span>T<span style=""color:red"">TCTAGA</span>TG<span style=""color:orange"">GCCGGC</span>
|-
|suffix with AgeI, SpeI, NotI and PstI:
|<span style=""color:orange"">ACCGGT</span><u>TAA</u>T<span style=""color:red"">AC<u>TAG</u><u>T</u></span><u>A</u><span style=""color:green""><u>G</u>CGGCCG</span><span style=""color:blue"">CTGCAG</span>
|}
Sites of restriction enzymes generating compatible overhangs have the same color:
<span style=""color:blue"">EcoRI</span> and <span style=""color:blue"">PstI</span> in blue, <span style=""color:green"">NotI</span> in green, <span style=""color:red"">XbaI</span> and <span style=""color:red"">SpeI</span> in red, <span style=""color:orange"">NgoMIV</span> and <span style=""color:orange"">AgeI</span> in orange. Stop codons are underlined."
false
false
_1726_
4206
20062
9
In stock
false
The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
false
Florian Riemer
BBa_K1351030_sequence
1
aaggagagata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z