LOCUS BBa_K1352000 4493 bp DNA linear 22-Nov-2024 DEFINITION Ag43 with PstI sites removed under the control of pBad/araC promoter ACCESSION BBa_K1352000 VERSION BBa_K1352000.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..4356 /label=Ag43 CDS 1237..4356 /label=BBa_K1352010 stem_loop 4376..4419 /label=stem_loop misc_feature 4365..4444 /label=BBa_B0010 terminator 4365..4444 /label=BBa_B0010 stem_loop 4460..4479 /label=T7 TE misc_feature 4486..4486 /label=stop polyA_site 4480..4493 /label=polya misc_feature 4453..4493 /label=BBa_B0012 terminator 4453..4493 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc 1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc 1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc 1381 ccggtgctgg ctgctgacat cgttgtgcac ccgggagaaa ccgtgaacgg cggaacactg 1441 gcaaatcatg acaaccagat tgtcttcggt acgaccaacg gaatgaccat cagtaccggg 1501 ctggagtatg ggccggataa cgaggccaat accggcgggc aatgggtaca ggatggcgga 1561 acagccaaca aaacgactgt caccagtggt ggtcttcaga gagtgaaccc cggtggaagt 1621 gtctcagaca cggttatcag tgccggaggc ggacagagcc ttcagggacg ggctgtgaac 1681 accacgctga atggtggcga acagtggatg catgaggggg cgatagccac aggaaccgtc 1741 attaatgata agggctggca ggtcgtcaag cccggtacag tggcaacgga taccgttgtt 1801 aataccgggg cggaaggggg accggatgca gaaaacggtg ataccgggca gtttgttcgc 1861 ggggatgccg tacgcacaac catcaataaa aacggtcgcc agattgtgag agctgaagga 1921 acggcaaata ccactgtggt ttatgccggc ggcgaccaga ctgtacatgg tcacgcactg 1981 gataccacgc tgaatggggg ataccagtat gtgcacaacg gcggtacagc gtctgacact 2041 gttgtgaaca gtgacggctg gcagattgtc aaaaacgggg gtgtggccgg gaataccacc 2101 gttaatcaga agggcagact gcaagtggac gccggtggta cagccacgaa tgtcaccctg 2161 aagcagggcg gcgcactggt taccagtacg gctgcaaccg ttaccggcat aaaccgcctg 2221 ggagcattct ctgttgtgga gggtaaagct gataatgtcg tactggaaaa tggcggacgc 2281 ctggatgtgc tgaccggaca cacagccact aatacccgcg tggatgatgg cggaacgctg 2341 gatgtccgca acggtggcac cgccaccacc gtatccatgg gaaatggcgg tgtactgctg 2401 gccgattccg gtgccgctgt cagtggtacc cggagcgacg gaaaggcatt cagtatcgga 2461 ggcggtcagg cggatgccct gatgctggaa aaaggcagtt cattcacgct gaacgccggt 2521 gatacggcca cggataccac ggtaaatggc ggactgttca ccgccagggg cggcacactg 2581 gcgggcacca ccacgctgaa taacggcgcc atacttaccc tttccgggaa gacggtgaac 2641 aacgataccc tgaccatccg tgaaggcgat gcactcctgc aaggaggctc tctcaccggt 2701 aacggcagcg tggaaaaatc aggaagtggc acactcactg tcagcaacac cacactcacc 2761 cagaaagccg tcaacctgaa tgaaggcacg ctgacgctga acgacagtac cgtcaccacg 2821 gatgtcattg ctcagcgcgg tacagccctg aagctgaccg gcagcactgt gctgaacggt 2881 gccattgacc ccacgaatgt cactctcgcc tccggtgcca cctggaatat ccccgataac 2941 gccacggtgc agtcggtggt ggatgacctc agccatgccg gacagattca tttcacctcc 3001 acccgcacag ggaagttcgt accggcaacc ctgaaagtga aaaacctgaa cggacagaat 3061 ggcaccatca gcctgcgtgt acgcccggat atggcacaga acaatgctga cagactggtc 3121 attgacggcg gcagggcaac cggaaaaacc atcctgaacc tggtgaacgc cggcaacagt 3181 gcgtcggggc tggcgaccag cggtaagggt attcaggtgg tggaagccat taacggtgcc 3241 accacggagg aaggggcctt tgtccagggg aacaggctgc aagccggtgc ctttaactac 3301 tccctcaacc gggacagtga tgagagctgg tatctgcgca gtgaaaatgc ttatcgtgca 3361 gaagtccccc tgtatgcctc catgctgaca caggcaatgg actatgaccg gattgtggca 3421 ggctcccgca gccatcagac cggtgtaaat ggtgaaaaca acagcgtccg tctcagcatt 3481 cagggcggtc atctcggtca cgataacaat ggcggtattg cccgtggggc cacgccggaa 3541 agcagcggca gctatggatt cgtccgtctg gagggtgacc tgatgagaac agaggttgcc 3601 ggtatgtctg tgaccgcggg ggtatatggt gctgctggcc attcttccgt tgatgttaag 3661 gatgatgacg gctcccgtgc cggcacggtc cgggatgatg ccggcagcct gggcggatac 3721 ctgaatctgg tacacacgtc ctccggcctg tgggctgaca ttgtggcaca gggaacccgc 3781 cacagcatga aagcgccatc ggacaataac gacttccgcg cccggggctg gggctggctg 3841 ggctcactgg aaaccggtct gcccttcagt atcactgaca acctgatgct ggagccacaa 3901 ctgcaatata cctggcaggg actttccctg gatgacggta aggacaacgc cggttatgtg 3961 aagttcgggc atggcagtgc acaacatgtg cgtgccggtt tccgtctggg cagccacaac 4021 gatatgacct ttggcgaagg cacctcatcc cgtgcccccc tgcgtgacag tgcaaaacac 4081 agtgtgagtg aattaccggt gaactggtgg gtacagcctt ctgttatccg caccttcagc 4141 tcccggggag atatgcgtgt ggggacttcc actgcgggca gcgggatgac gttctctccc 4201 tcacagaatg gcacatcact ggacctgcaa gccggactgg aagcccgtgt ccgggaaaat 4261 atcaccctgg gcgttcaggc cggttatgcc cacagcgtca gcggcagcag cgctgaaggg 4321 tataacggtc aggccacact gaatgtgacc ttctgatact agagccaggc atcaaataaa 4381 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4441 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //