LOCUS BBa_K1352001 4529 bp DNA linear 22-Nov-2024 DEFINITION Ag43+FLAG+MCS under the control of pBAD promoter ACCESSION BBa_K1352001 VERSION BBa_K1352001.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..4392 /label=Ag43+FLAG+MCS misc_feature 1678..1713 /label=BglII-FLAG-HindIII CDS 1237..4392 /label=BBa_K1352011 stem_loop 4412..4455 /label=stem_loop misc_feature 4401..4480 /label=BBa_B0010 terminator 4401..4480 /label=BBa_B0010 stem_loop 4496..4515 /label=T7 TE misc_feature 4522..4522 /label=stop polyA_site 4516..4529 /label=polya misc_feature 4489..4529 /label=BBa_B0012 terminator 4489..4529 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc 1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc 1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc 1381 ccggtgctgg ctgctgacat cgttgtgcac ccgggagaaa ccgtgaacgg cggaacactg 1441 gcaaatcatg acaaccagat tgtcttcggt acgaccaacg gaatgaccat cagtaccggg 1501 ctggagtatg ggccggataa cgaggccaat accggcgggc aatgggtaca ggatggcgga 1561 acagccaaca aaacgactgt caccagtggt ggtcttcaga gagtgaaccc cggtggaagt 1621 gtctcagaca cggttatcag tgccggaggc ggacagagcc ttcagggacg ggctgtgaga 1681 tctgattata aagatgatga tgataaaaag cttaacacca cgctgaatgg tggcgaacag 1741 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc 1801 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg 1861 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc 1921 aataaaaacg gtcgccagat tgtgagagct gaaggaacgg caaataccac tgtggtttat 1981 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac 2041 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag 2101 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcaa 2161 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc 2221 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt 2281 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca 2341 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc 2401 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt 2461 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg 2521 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta 2581 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac 2641 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa 2701 ggcgatgcac tcctgcaagg aggctctctc accggtaacg gcagcgtgga aaaatcagga 2761 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa 2821 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca 2881 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact 2941 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat 3001 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg 3061 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc 3121 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga 3181 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt 3241 aagggtattc aggtggtgga agccattaac ggtgccacca cggaggaagg ggcctttgtc 3301 caggggaaca ggctgcaagc cggtgccttt aactactccc tcaaccggga cagtgatgag 3361 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg 3421 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt 3481 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat 3541 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc 3601 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta 3661 tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc 3721 acggtccggg atgatgccgg cagcctgggc ggatacctga atctggtaca cacgtcctcc 3781 ggcctgtggg ctgacattgt ggcacaggga acccgccaca gcatgaaagc gccatcggac 3841 aataacgact tccgcgcccg gggctggggc tggctgggct cactggaaac cggtctgccc 3901 ttcagtatca ctgacaacct gatgctggag ccacaactgc aatatacctg gcagggactt 3961 tccctggatg acggtaagga caacgccggt tatgtgaagt tcgggcatgg cagtgcacaa 4021 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc 4081 tcatcccgtg cccccctgcg tgacagtgca aaacacagtg tgagtgaatt accggtgaac 4141 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggagatat gcgtgtgggg 4201 acttccactg cgggcagcgg gatgacgttc tctccctcac agaatggcac atcactggac 4261 ctgcaagccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt 4321 tatgcccaca gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat 4381 gtgaccttct gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 4441 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 4501 accttcgggt gggcctttct gcgtttata //