LOCUS BBa_K1352002 4427 bp DNA linear 03-May-2024 DEFINITION Ag43+FLAG+MCS(-)beta-hairpins under pBAD promoter ACCESSION BBa_K1352002 VERSION BBa_K1352002.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..4290 /label=Ag43+FLAG+MCS(-)beta-hairpins misc_feature 1678..1713 /label=BglII+FLAG+HindIII CDS 1237..4290 /label=BBa_K1352012 stem_loop 4310..4353 /label=stem_loop misc_feature 4299..4378 /label=BBa_B0010 terminator 4299..4378 /label=BBa_B0010 stem_loop 4394..4413 /label=T7 TE misc_feature 4420..4420 /label=stop polyA_site 4414..4427 /label=polya misc_feature 4387..4427 /label=BBa_B0012 terminator 4387..4427 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc 1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc 1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc 1381 ccggtgctgg ctgctgacat cgttgtgcac ccgggagaaa ccgtgaacgg cggaacactg 1441 gcaaatcatg acaaccagat tgtcttcggt acgaccaacg gaatgaccat cagtaccggg 1501 ctggagtatg ggccggataa cgaggccaat accggcgggc aatgggtaca ggatggcgga 1561 acagccaaca aaacgactgt caccagtggt ggtcttcaga gagtgaaccc cggtggaagt 1621 gtctcagaca cggttatcag tgccggaggc ggacagagcc ttcagggacg ggctgtgaga 1681 tctgattata aagatgatga tgataaaaag cttaacacca cgctgaatgg tggcgaacag 1741 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc 1801 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg 1861 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc 1921 aataaaaacg gtcgccagat tgtgagagct gaaggaacgg caaataccac tgtggtttat 1981 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac 2041 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag 2101 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcaa 2161 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc 2221 agtacgggta aagctgataa tgtcgtactg gaaaatggcg gacgcctgga tgtgctgacc 2281 ggacacacag ccactaatac ccgcgtggat gatggcggaa cgctggatgt ccgcaacggt 2341 ggcaccgcca ccaccgtatc catgggaaat ggcggtgtac tgctggccga ttccggcggt 2401 caggcggatg ccctgatgct ggaaaaaggc agttcattca cgctgaacgc cggtgatacg 2461 gccacggata ccacggtaaa tggcggactg ttcaccgcca ggggcggcac actggcgggc 2521 accaccacgc tgaataacgg cgccatactt accctttccg ggaagacggt gaacaacgat 2581 accctgacca tccgtgaagg cgatgcactc ctgcaaggag gctctctcac cggtaacggc 2641 agcgtggaaa aatcaggaag tggcacactc actgtcagca acaccacact cacccagaaa 2701 gccgtcaacc tgaatgaagg cacgctgacg ctgaacgaca gtaccgtcac cacggatgtc 2761 attgctcagc gcggtacagc cctgaagctg accggcagca ctgtgctgaa cggtgccatt 2821 gaccccacga atgtcactct cgcctccggt gccacctgga atatccccga taacgccacg 2881 gtgcagtcgg tggtggatga cctcagccat gccggacaga ttcatttcac ctccacccgc 2941 acagggaagt tcgtaccggc aaccctgaaa gtgaaaaacc tgaacggaca gaatggcacc 3001 atcagcctgc gtgtacgccc ggatatggca cagaacaatg ctgacagact ggtcattgac 3061 ggcggcaggg caaccggaaa aaccatcctg aacctggtga acgccggcaa cagtgcgtcg 3121 gggctggcga ccagcggtaa gggtattcag gtggtggaag ccattaacgg tgccaccacg 3181 gaggaagggg cctttgtcca ggggaacagg ctgcaagccg gtgcctttaa ctactccctc 3241 aaccgggaca gtgatgagag ctggtatctg cgcagtgaaa atgcttatcg tgcagaagtc 3301 cccctgtatg cctccatgct gacacaggca atggactatg accggattgt ggcaggctcc 3361 cgcagccatc agaccggtgt aaatggtgaa aacaacagcg tccgtctcag cattcagggc 3421 ggtcatctcg gtcacgataa caatggcggt attgcccgtg gggccacgcc ggaaagcagc 3481 ggcagctatg gattcgtccg tctggagggt gacctgatga gaacagaggt tgccggtatg 3541 tctgtgaccg cgggggtata tggtgctgct ggccattctt ccgttgatgt taaggatgat 3601 gacggctccc gtgccggcac ggtccgggat gatgccggca gcctgggcgg atacctgaat 3661 ctggtacaca cgtcctccgg cctgtgggct gacattgtgg cacagggaac ccgccacagc 3721 atgaaagcgc catcggacaa taacgacttc cgcgcccggg gctggggctg gctgggctca 3781 ctggaaaccg gtctgccctt cagtatcact gacaacctga tgctggagcc acaactgcaa 3841 tatacctggc agggactttc cctggatgac ggtaaggaca acgccggtta tgtgaagttc 3901 gggcatggca gtgcacaaca tgtgcgtgcc ggtttccgtc tgggcagcca caacgatatg 3961 acctttggcg aaggcacctc atcccgtgcc cccctgcgtg acagtgcaaa acacagtgtg 4021 agtgaattac cggtgaactg gtgggtacag ccttctgtta tccgcacctt cagctcccgg 4081 ggagatatgc gtgtggggac ttccactgcg ggcagcggga tgacgttctc tccctcacag 4141 aatggcacat cactggacct gcaagccgga ctggaagccc gtgtccggga aaatatcacc 4201 ctgggcgttc aggccggtta tgcccacagc gtcagcggca gcagcgctga agggtataac 4261 ggtcaggcca cactgaatgt gaccttctga tactagagcc aggcatcaaa taaaacgaaa 4321 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4381 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //