LOCUS       BBa_K1355002            2075 bp    DNA     linear       22-Dec-2024
DEFINITION  Mercury ions detector device
ACCESSION   BBa_K1355002
VERSION     BBa_K1355002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=Trp Terminator
     CDS             44..480
                     /label=MerR
     promoter        486..514
                     /label=Bidirecional promoter
     promoter        510..538
                     /label=Bidirecional promoter
     CDS             551..900
                     /label=MerT
     RBS             901..907
                     /label=RBS
     CDS             914..1189
                     /label=MerP
     RBS             490..496
                     /label=RBS
     RBS             537..543
                     /label=RBS
     misc_feature    1..1189
                     /label=BBa_K1355001
     RBS             1198..1212
                     /label=RBS-1\Strong
     RBS             1205..1209
                     /label=RBS
     misc_feature    1198..1212
                     /label=BBa_B0030
     RBS             1198..1212
                     /label=BBa_B0030
     CDS             1219..1938
                     /label=GFP protein
     CDS             1219..1938
                     /label=BBa_E0040
     stem_loop       1958..2001
                     /label=stem_loop
     misc_feature    1947..2026
                     /label=BBa_B0010
     terminator      1947..2026
                     /label=BBa_B0010
     stem_loop       2042..2061
                     /label=T7 TE
     misc_feature    2068..2068
                     /label=stop
     polyA_site      2062..2075
                     /label=polya
     misc_feature    2035..2075
                     /label=BBa_B0012
     terminator      2035..2075
                     /label=BBa_B0012
     misc_feature    1198..2075
                     /label=BBa_E0840
ORIGIN
        1 aaagaaagtt aaaatgccgc cagcggaact ggcggctgtg ggactaaggc atagctgacc
       61 ttgccaggcc tgcttcgccc tgtagtgacg cgatcaacgg gcaggaaaca ttcccctttc
      121 gtgcatggca ggcgcacacg agttcagaca gcacggtttc catgcgcgcc aagtcggcca
      181 tcttctcgcg cacgtccttg agcttgtgtt cggccaggct gctggcctcc tcgcagtggg
      241 tgccatcgtc gagccgcaac agctcggcaa tctcgtccag actgaacccc agccgctgtg
      301 ccgatttcac gaatttcacc cgaaccacgt ccgcctcccc atagcggcgg atgctgccgt
      361 aaggcttgtc cggttcccgc aacaggccct tgcgctgata gaagcggatt gtctccacgt
      421 tgaccccggc cgccttggca aaaacgccaa tggtcaggtt ttccaaatta ttttccatat
      481 cgcttgactc cgtacatgag tacggaagta aggttacgct atccaatcca aattcaaaag
      541 ggccaacgta tgtctgaacc acaaaacggg cgcggtgcgc tcttcgccgg cgggctggcc
      601 gccattcttg catcgacctg ctgcctgggg ccgctagtac tggtcgccct gggcttctcc
      661 ggtgcttgga tcggcaacct gacggtgctg gaaccctatc gaccgttgtt catcggcgcg
      721 gcgctagtgg cgctgttctt cgcctggaag cggatttacc ggcccgtgca ggcatgcaag
      781 ccaggtgagg tctgcgcgat tccgcaggtg cgcgccacct acaagctgat tttctggatc
      841 gtggccgtgc tggtcctggt cgcgcttgga tttccctatg tcgttccatt tttctattaa
      901 ccaggagttc atcatgaaga aactgtttgc ctcccttgcc ctcgccgccg ctgttgcccc
      961 ggtgtgggcc gctacccaga ccgtcacgct agcggttccc ggcatgactt gcgccgcctg
     1021 cccgatcaca gtcaagaaag cgctctccaa ggtcgaaggc gtgagcaagg tcgatgtggg
     1081 cttcgagaag cgcgaggccg tcgtcacttt tgacgacacc aaggccagcg tacagaagct
     1141 gaccaaggcc accgcagacg ccggctatcc gtccagcgtc aagcagtgat actagagatt
     1201 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
     1261 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     1321 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     1381 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
     1441 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     1501 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     1561 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     1621 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1681 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     1741 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1801 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1861 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1921 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1981 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2041 tggctcacct tcgggtgggc ctttctgcgt ttata
//