LOCUS BBa_K1356000 1589 bp DNA linear 23-Dec-2024 DEFINITION Alpha Amylase with TolB Signaling Sequence and PstI Site Removed ACCESSION BBa_K1356000 VERSION BBa_K1356000.1 FEATURES Location/Qualifiers misc_feature 1..101 /label=DsbA Signaling Sequence CDS 102..1589 /label=Alpha Amylase misc_feature 977..977 /label=Mutated PstI Restriction Site for Removal ORIGIN 1 atccgcgcga gcaggggaaa ttgacggaaa acctataccg gccagcaaca ggaatgccgt 61 aagcagccgc agtaaattca tatgcgtaat cccacgctga tatgcgtaat cccacgctgt 121 tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc 181 gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag 241 gcgcatcggg cgggtattcg gtcggctacg actcctatga tttatttgat ttaggcgagt 301 ttgatcagaa aggcagcatc cctactaaat atggcgataa agcacaactg ctggccgcca 361 ttgatgctct gaaacgtaat gacattgcgg tgctgttgga tgtggtagtc aaccacaaaa 421 tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc 481 aaattgacga agaaatcatt gagtgtgaag gctggacgcg ttacaccttc cccgcccgtg 541 ccgggcaata ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg 601 aaaaccctga cgaagatggc atttttaaaa ttgttaacga ctacaccggc gaaggctgga 661 acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag aatatcgatt 721 ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa 781 cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccagcctgg ttttataaag 841 agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg gcggagtact 901 ggtcgcatga agttgataag ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc 961 tgtttgatgc gccgctccag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg 1021 acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc 1081 tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc gaaccgtggt 1141 ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct tcggtattct 1201 atccggacct ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa acctatccga 1261 tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc 1321 acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca 1381 ccgacgaatt tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca 1441 ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg aaccggcaag 1501 agagagtagt gaccgacgaa aacggcgaag caaccttctt ttgcaacggc ggcagcgtca 1561 gcgtgtgggt tatcgaagag gtgatttaa //