LOCUS BBa_K1357003 1124 bp DNA linear 31-Oct-2024 DEFINITION IPTG Inducible NhaR Expression Construct ACCESSION BBa_K1357003 VERSION BBa_K1357003.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 1..75 /label=BBa_K215000 CDS 82..987 /label=BBa_K1357002 stem_loop 1007..1050 /label=stem_loop misc_feature 996..1075 /label=BBa_B0010 terminator 996..1075 /label=BBa_B0010 stem_loop 1091..1110 /label=T7 TE misc_feature 1117..1117 /label=stop polyA_site 1111..1124 /label=polya misc_feature 1084..1124 /label=BBa_B0012 terminator 1084..1124 /label=BBa_B0012 terminator 996..1124 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcatg tctcatatca attacaacca cttgtattac 121 ttctggcatg tctataaaga aggttccgtg gttggcgcag cggaggcgct ttatttaact 181 ccacaaacca ttaccggaca gattcgagcg ctggaagacg ccctgcaagc gaaattattt 241 aaacgcaagg gacgtggtct cgaacccagc gagctgggag aactggtcta tcgctatgcc 301 gataaaatgt tcaccttaag ccaggaaatg ctggatattg tgaactatcg caaagaatcc 361 aatttattgt ttgacgttgg cgtggctgat gcactttcca aacgcctggt cagtagcgta 421 cttaacgccg cagtggtaga aggcgagccc attcatcttc gctgcttcga atccacccac 481 gaaatgctgc tggagcaatt aagtcagcat aaactggaga tgatcatttc tgactgtccg 541 atagactcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt 601 ttctggtgta caaatccacc accagaaaaa ccgttcccgg cttgtctgga agaacggcga 661 cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc 721 cagggattaa acgtagaaat cctcggcgag tttgatgatg ccgctttgat gaaagctttt 781 ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga cttttatgcc 841 gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta ccatgctatt 901 tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac ggattattct 961 gcgcttttta gtccagcggt gcgttaatac tagagccagg catcaaataa aacgaaaggc 1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //