LOCUS       BBa_K1357004            1104 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive NhaR Expression Device
ACCESSION   BBa_K1357004
VERSION     BBa_K1357004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..967
                     /label=BBa_K1357002
     stem_loop       987..1030
                     /label=stem_loop
     misc_feature    976..1055
                     /label=BBa_B0010
     terminator      976..1055
                     /label=BBa_B0010
     stem_loop       1071..1090
                     /label=T7 TE
     misc_feature    1097..1097
                     /label=stop
     polyA_site      1091..1104
                     /label=polya
     misc_feature    1064..1104
                     /label=BBa_B0012
     terminator      1064..1104
                     /label=BBa_B0012
     terminator      976..1104
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagcatg tctcatatca attacaacca cttgtattac ttctggcatg tctataaaga
      121 aggttccgtg gttggcgcag cggaggcgct ttatttaact ccacaaacca ttaccggaca
      181 gattcgagcg ctggaagacg ccctgcaagc gaaattattt aaacgcaagg gacgtggtct
      241 cgaacccagc gagctgggag aactggtcta tcgctatgcc gataaaatgt tcaccttaag
      301 ccaggaaatg ctggatattg tgaactatcg caaagaatcc aatttattgt ttgacgttgg
      361 cgtggctgat gcactttcca aacgcctggt cagtagcgta cttaacgccg cagtggtaga
      421 aggcgagccc attcatcttc gctgcttcga atccacccac gaaatgctgc tggagcaatt
      481 aagtcagcat aaactggaga tgatcatttc tgactgtccg atagactcta cgcagcagga
      541 aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt ttctggtgta caaatccacc
      601 accagaaaaa ccgttcccgg cttgtctgga agaacggcga cttttgattc ctgggcgacg
      661 ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc cagggattaa acgtagaaat
      721 cctcggcgag tttgatgatg ccgctttgat gaaagctttt ggtgcgatgc acaatgcaat
      781 cttcgttgcc ccaacgcttt atgcatatga cttttatgcc gataaaactg tcgtagaaat
      841 tggtcgcgtc gagaatgtga tggaagagta ccatgctatt tttgctgagc ggatgattca
      901 gcacccggcg gtacagcgaa tctgcaatac ggattattct gcgcttttta gtccagcggt
      961 gcgttaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1021 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1081 cgggtgggcc tttctgcgtt tata
//