BBa_K136007
1
BBa_K136007
flgB promotor (RBS WT)
2008-10-24T11:00:00Z
2015-05-08T01:10:02Z
This part comes from the genome of E.coli K12 strain MG1655.
Promoter of flgB gene from E. coli flagella
flgB is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).
Its promoter is consitutively repressed.
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
true
false
_211_
0
3262
9
Discontinued
false
>forward primer
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
>reverse primer
GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
false
Kok-Phen YAN
annotation1986992
1
atg
range1986992
1
198
200
annotation1986990
1
flhDC binding site
range1986990
1
98
113
annotation1986993
1
transcription initiation site
range1986993
1
173
173
annotation1986991
1
flhDC binding site
range1986991
1
124
139
BBa_K136007_sequence
1
acagtatcgcgatgatcgccacgctacgttttattatcagcattttcgcccccagccatttctacaacgtgaattgtacctgtccgcaatgaccatcaacggcataaatagcgacccattttgcgtttattccgccgataacgcgcgcgtaaaggcatttaagctgatggcagaattttgatacctgcggaggagatatgct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z