BBa_K136008
1
BBa_K136008
flhB promoter followed by its natural RBS
2008-10-24T11:00:00Z
2015-05-08T01:10:02Z
This part comes from the genome of E.coli K12 strain MG1655.
Promoter of flhB gene from E. coli flagella
flhB is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).
Its promoter is consitutively repressed.
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
false
false
_211_
0
3262
9
It's complicated
false
>forward primer
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
>reverse primer
GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
false
Kok-Phen YAN
annotation1986994
1
flhDC binding site
range1986994
1
79
94
annotation1999346
1
natural flhB RBS
range1999346
1
166
173
annotation1986996
1
transcription initiation site
range1986996
1
153
153
annotation1986995
1
flhDC binding site
range1986995
1
106
121
annotation1999345
1
GTG
range1999345
1
178
180
BBa_K136008_sequence
1
ccacgtcatatcaggcggtctgataaggcgatgacgccgcatccgacaaacgcacactatgcttgatgtgtggcagcaaaagccctaaatcccgcctgttttgccccttactcaaaccattgaacgctttgcgctctggcatcattcacgcttaatactctttccaggattggcgacgtgtctgacgagagcgacgacaaaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z