LOCUS       BBa_K136012             1093 bp    DNA     linear       29-Nov-2024
DEFINITION  flgA promoter - YFP Tripart (LVA+)
ACCESSION   BBa_K136012
VERSION     BBa_K136012.1  
FEATURES             Location/Qualifiers
     misc_feature    63..78
                     /label=flhDC binding site 
     misc_feature    89..104
                     /label=flhDC binding site
     misc_feature    160..162
                     /label=ATG
     misc_feature    138..138
                     /label=transcription initiation
     RBS             146..154
                     /label=nautral flgA RBS
     promoter        1..202
                     /label=BBa_K136006
     misc_feature    215..218
                     /label=conserved
     RBS             211..222
                     /label=BBa_B0034
     CDS             229..990
                     /label=YFP (LVA)
     misc_feature    985..990
                     /label=2
     misc_feature    946..984
                     /label=SsrA
     misc_feature    470..470
                     /label=A (Q->L)
     misc_feature    229..990
                     /label=BBa_E0032
     misc_feature    229..990
                     /label=BBa_E0032
     stem_loop       1006..1025
                     /label=T7 TE
     misc_feature    1032..1032
                     /label=stop
     polyA_site      1026..1039
                     /label=polya
     misc_feature    999..1039
                     /label=BBa_B0012
     terminator      999..1039
                     /label=BBa_B0012
     stem_loop       1060..1082
                     /label=stem_loop
     misc_feature    1048..1093
                     /label=BBa_B0011
     terminator      1048..1093
                     /label=BBa_B0011
ORIGIN
        1 agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc
       61 gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg tcattgcgga
      121 caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgataat aaaacgtagc
      181 gtggcgatca tcgcgatact gttactagag aaagaggaga aatactagat ggtgagcaag
      241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      361 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      421 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
      481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      661 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      781 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
      841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
      961 gacgaaaact acgctttagt agcttaataa tactagagtc acactggctc accttcgggt
     1021 gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc
     1081 ttttttatta ttt
//