##gff-version 3
##sequence-region BBa_K136012 1 1093
BBa_K136012	.	engineered_region	229	990	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_K136012	.	sequence_alteration	470	470	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_K136012	.	engineered_region	229	990	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_K136012	.	tag	946	984	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_K136012	.	stop_codon	985	990	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_K136012	.	CDS	229	990	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_K136012	.	terminator	1048	1093	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K136012	.	engineered_region	1048	1093	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K136012	.	stem_loop	1060	1082	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K136012	.	terminator	999	1039	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K136012	.	stem_loop	1006	1025	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136012	.	stop_codon	1032	1032	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136012	.	polyA_site	1026	1039	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136012	.	engineered_region	999	1039	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136012	.	ribosome_entry_site	211	222	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K136012	.	conserved_region	215	218	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K136012	.	promoter	1	202	.	+	0	ID=BBa_K136006;Name=BBa_K136006
BBa_K136012	.	non_covalent_binding_site	89	104	.	+	0	ID=annotation1986987;Name=flhDC binding site;Parent=BBa_K136006
BBa_K136012	.	start_codon	138	138	.	+	0	ID=annotation1986989;Name=transcription initiation;Parent=BBa_K136006
BBa_K136012	.	ribosome_entry_site	146	154	.	+	0	ID=annotation1999347;Name=nautral flgA RBS;Parent=BBa_K136006
BBa_K136012	.	start_codon	160	162	.	+	0	ID=annotation1986988;Name=ATG;Parent=BBa_K136006
BBa_K136012	.	non_covalent_binding_site	63	78	.	+	0	ID=annotation1986986;Name=flhDC binding site ;Parent=BBa_K136006
>BBa_K136012
agcatatctcctccgcaggtatcaaaattctgccatcagcttaaatgcctttacgcgcgcgttatcggcggaataaacg
caaaatgggtcgctatttatgccgttgatggtcattgcggacaggtacaattcacgttgtagaaatggctgggggcgaa
aatgctgataataaaacgtagcgtggcgatcatcgcgatactgttactagagaaagaggagaaatactagatggtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcg
tgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgt
gccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcac
gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaaga
cccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacgg
caacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggc
atcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccc
ccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacga
gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagagg
cctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt