LOCUS       BBa_K136019              921 bp    DNA     linear       23-Dec-2024
DEFINITION  weak promoter - GFP tripart
ACCESSION   BBa_K136019
VERSION     BBa_K136019.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..784
                     /label=GFP protein
     CDS             65..784
                     /label=BBa_E0040
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagattaaag aggagaaata
       61 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      121 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      181 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      241 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      301 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      361 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      421 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      481 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      541 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      601 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      661 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      721 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat a
//