LOCUS       BBa_I2010                936 bp    DNA     linear       22-Nov-2024
DEFINITION  A_Receiver Device (R1075, B0034.C0079.B0015)
ACCESSION   BBa_I2010
VERSION     BBa_I2010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..735
                     /label=lasR
     misc_feature    736..768
                     /label=LVA
     misc_feature    498..498
                     /label=G
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0079
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt
       61 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag
      121 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc
      181 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg
      241 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag
      301 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg
      361 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg
      421 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc
      481 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg
      541 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt
      601 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat
      661 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg
      721 ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttata
//
LOCUS       BBa_K136023              979 bp    DNA     linear       22-Nov-2024
DEFINITION  strong promoter - RBS - lasR - double terminator
ACCESSION   BBa_K136023
VERSION     BBa_K136023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..778
                     /label=lasR
     misc_feature    779..811
                     /label=LVA
     misc_feature    541..541
                     /label=G
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0079
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_K136026
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcgc
      781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttata
//