LOCUS       BBa_K136027             1082 bp    DNA     linear       08-May-2024
DEFINITION  lasR promoter - ECFP tripart LVA
ACCESSION   BBa_K136027
VERSION     BBa_K136027.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     CDS             184..945
                     /label=CFP (LVA)
     misc_feature    902..939
                     /label=SsrA
     misc_feature    940..945
                     /label=2
     misc_feature    252..252
                     /label=A
     misc_feature    184..945
                     /label=BBa_E0022
     misc_feature    184..945
                     /label=BBa_E0022
     stem_loop       965..1008
                     /label=stem_loop
     misc_feature    954..1033
                     /label=BBa_B0010
     terminator      954..1033
                     /label=BBa_B0010
     stem_loop       1049..1068
                     /label=T7 TE
     misc_feature    1075..1075
                     /label=stop
     polyA_site      1069..1082
                     /label=polya
     misc_feature    1042..1082
                     /label=BBa_B0012
     terminator      1042..1082
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      241 gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      301 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      361 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg
      421 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      481 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      541 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      601 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag
      661 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      721 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      781 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      841 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      901 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca
      961 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1021 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1081 ta
//