LOCUS       BBa_K136029             1043 bp    DNA     linear       29-Nov-2024
DEFINITION  lasR promoter - GFP tripart
ACCESSION   BBa_K136029
VERSION     BBa_K136029.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..906
                     /label=GFP protein
     CDS             187..906
                     /label=BBa_E0040
     stem_loop       926..969
                     /label=stem_loop
     misc_feature    915..994
                     /label=BBa_B0010
     terminator      915..994
                     /label=BBa_B0010
     stem_loop       1010..1029
                     /label=T7 TE
     misc_feature    1036..1036
                     /label=stop
     polyA_site      1030..1043
                     /label=polya
     misc_feature    1003..1043
                     /label=BBa_B0012
     terminator      1003..1043
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      901 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      961 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1021 gggtgggcct ttctgcgttt ata
//