##gff-version 3
##sequence-region BBa_K136035 1 1628
##sequence-region BBa_J24710 1 1628
BBa_K136035	.	engineered_region	730	1491	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_K136035	.	stop_codon	1486	1491	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K136035	.	tag	1448	1485	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K136035	.	point_mutation	798	798	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K136035	.	CDS	730	1491	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K136035	.	engineered_region	730	1491	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K136035	.	terminator	1588	1628	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K136035	.	polyA_site	1615	1628	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136035	.	stem_loop	1595	1614	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136035	.	stop_codon	1621	1621	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136035	.	engineered_region	1588	1628	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136035	.	terminator	1500	1579	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K136035	.	stem_loop	1511	1554	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K136035	.	engineered_region	1500	1579	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K136035	.	ribosome_entry_site	712	723	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K136035	.	conserved_region	716	719	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K136035	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K136035	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K136035	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K136035	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K136035	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K136035	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24710	.	engineered_region	712	1628	.	+	0	ID=BBa_J24703;Name=BBa_J24703
BBa_J24710	.	ribosome_entry_site	712	723	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24703
BBa_J24710	.	conserved_region	716	719	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24710	.	terminator	1500	1579	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24703
BBa_J24710	.	stem_loop	1511	1554	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24710	.	engineered_region	1500	1579	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24710	.	terminator	1588	1628	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24703
BBa_J24710	.	polyA_site	1615	1628	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24710	.	stem_loop	1595	1614	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24710	.	stop_codon	1621	1621	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24710	.	engineered_region	1588	1628	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24710	.	engineered_region	730	1491	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_J24703
BBa_J24710	.	stop_codon	1486	1491	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J24710	.	tag	1448	1485	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J24710	.	point_mutation	798	798	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J24710	.	CDS	730	1491	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J24710	.	engineered_region	730	1491	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J24710	.	engineered_region	1	703	.	+	0	ID=BBa_J24688;Name=BBa_J24688
BBa_J24710	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24688
BBa_J24710	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24710	.	CDS	19	703	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24688
BBa_J24710	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24710	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24710	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K136035
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagc
cgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctat
atcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata
>BBa_J24710
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagc
cgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctat
atcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata