LOCUS       BBa_K136036             1589 bp    DNA     linear       07-May-2025
DEFINITION  rbs TetR - GFP tripart
ACCESSION   BBa_K136036
VERSION     BBa_K136036.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     misc_feature    891..894
                     /label=conserved
     RBS             887..898
                     /label=BBa_B0034
     CDS             908..1524
                     /label=tetR
     misc_feature    1525..1558
                     /label=SsrA
     misc_feature    1565..1589
                     /label=Help:Barcodes
     CDS             905..1564
                     /label=BBa_C0040
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaaag aggagaaata
      901 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      961 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     1021 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     1081 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     1141 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     1201 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     1261 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     1321 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     1381 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     1441 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     1501 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
     1561 ataacactga tagtgctagt gtagatcac
//