LOCUS       BBa_K136038             1589 bp    DNA     linear       22-Nov-2024
DEFINITION  rbs TetR - GFP tripart
ACCESSION   BBa_K136038
VERSION     BBa_K136038.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     RBS             712..726
                     /label=RBS-1\Strong
     RBS             719..723
                     /label=RBS
     misc_feature    712..726
                     /label=BBa_B0030
     RBS             712..726
                     /label=BBa_B0030
     CDS             733..1452
                     /label=GFP protein
     CDS             733..1452
                     /label=BBa_E0040
     stem_loop       1472..1515
                     /label=stem_loop
     misc_feature    1461..1540
                     /label=BBa_B0010
     terminator      1461..1540
                     /label=BBa_B0010
     stem_loop       1556..1575
                     /label=T7 TE
     misc_feature    1582..1582
                     /label=stop
     polyA_site      1576..1589
                     /label=polya
     misc_feature    1549..1589
                     /label=BBa_B0012
     terminator      1549..1589
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gattaaagag
      721 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      781 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      841 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      901 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      961 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1021 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1081 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1141 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1201 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1261 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1321 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1381 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1441 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1501 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1561 accttcgggt gggcctttct gcgtttata
//