LOCUS       BBa_K136039             1079 bp    DNA     linear       25-Nov-2024
DEFINITION  fliL promoter + WT RBS (K136009) - ECFP tripart + LVA (E0422)
ACCESSION   BBa_K136039
VERSION     BBa_K136039.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     misc_feature    167..170
                     /label=conserved
     RBS             163..174
                     /label=BBa_B0034
     CDS             181..942
                     /label=CFP (LVA)
     misc_feature    899..936
                     /label=SsrA
     misc_feature    937..942
                     /label=2
     misc_feature    249..249
                     /label=A
     misc_feature    181..942
                     /label=BBa_E0022
     misc_feature    181..942
                     /label=BBa_E0022
     stem_loop       962..1005
                     /label=stem_loop
     misc_feature    951..1030
                     /label=BBa_B0010
     terminator      951..1030
                     /label=BBa_B0010
     stem_loop       1046..1065
                     /label=T7 TE
     misc_feature    1072..1072
                     /label=stop
     polyA_site      1066..1079
                     /label=polya
     misc_feature    1039..1079
                     /label=BBa_B0012
     terminator      1039..1079
                     /label=BBa_B0012
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag agaaagagga gaaatactag
      181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      241 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      301 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      361 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      481 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      601 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      661 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      721 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      781 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      841 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg
      901 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca
      961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1021 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//