##gff-version 3
##sequence-region BBa_K136039 1 1079
BBa_K136039	.	ribosome_entry_site	163	174	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K136039	.	conserved_region	167	170	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K136039	.	promoter	1	154	.	+	0	ID=BBa_K136009;Name=BBa_K136009
BBa_K136039	.	ribosome_entry_site	134	141	.	+	0	ID=annotation1999344;Name=natural fliL RBS;Parent=BBa_K136009
BBa_K136039	.	sequence_feature	127	127	.	+	0	ID=annotation1987001;Name=transcription initiation site;Parent=BBa_K136009
BBa_K136039	.	sequence_feature	116	116	.	+	0	ID=annotation1987003;Name=transcription initiation site;Parent=BBa_K136009
BBa_K136039	.	non_covalent_binding_site	70	85	.	+	0	ID=annotation1987004;Name=FlhDC binding site;Parent=BBa_K136009
BBa_K136039	.	non_covalent_binding_site	44	59	.	+	0	ID=annotation1987002;Name=FlhDC binding site;Parent=BBa_K136009
BBa_K136039	.	start_codon	150	152	.	+	0	ID=annotation1999343;Name=ATG;Parent=BBa_K136009
BBa_K136039	.	terminator	1039	1079	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K136039	.	stem_loop	1046	1065	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136039	.	engineered_region	1039	1079	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136039	.	stop_codon	1072	1072	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136039	.	polyA_site	1066	1079	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136039	.	engineered_region	181	942	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_K136039	.	tag	899	936	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K136039	.	CDS	181	942	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K136039	.	engineered_region	181	942	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K136039	.	point_mutation	249	249	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K136039	.	stop_codon	937	942	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K136039	.	terminator	951	1030	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K136039	.	stem_loop	962	1005	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K136039	.	engineered_region	951	1030	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K136039
caagggcgtgtaacaggcaacagcggcgttgatattttcgcctaacgtcagaggtagcaccgtaatccgcgtcttttcc
ccgctttgttgcgctcaagacgcaggataattagccgataagcagtagcgacacaggaagaccgcaacacatgactact
agagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctg
gacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctga
agttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgctt
cagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcg
agctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgt
ctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtg
cagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagca
cccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggat
cactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata