LOCUS       BBa_K136040             3158 bp    DNA     linear       28-Jun-2024
DEFINITION  pFlgA-RFP
ACCESSION   BBa_K136040
VERSION     BBa_K136040.1  
FEATURES             Location/Qualifiers
     misc_feature    63..78
                     /label=flhDC binding site 
     misc_feature    89..104
                     /label=flhDC binding site
     misc_feature    160..162
                     /label=ATG
     misc_feature    138..138
                     /label=transcription initiation
     RBS             146..154
                     /label=nautral flgA RBS
     promoter        1..202
                     /label=BBa_K136006
     promoter        2938..2966
                     /label=beta-lactamase (ampR, bla) promoter
     CDS             237..917
                     /label=mRFP
     misc_feature    951..1079
                     /label=BBa_B0015
     misc_feature    1259..1941
                     /label=ColE1 origin
     CDS             2036..2896
                     /label=beta-lactamase ampicillin resistance marker
     stem_loop       962..1005
                     /label=rrnB terminator
     misc_feature    1262..1881
                     /label=pBR322 origin
     misc_feature    3137..3158
                     /label=BioBrick prefix
     misc_feature    212..217
                     /label=SpeI
     misc_feature    1095..1100
                     /label=PstI
     misc_feature    1088..1095
                     /label=NotI
     RBS             219..230
                     /label=RBS
     misc_feature    219..230
                     /label=BBa_B0034 RBS
     primer_bind     1158..1177
                     /label=Verification reverse (VR) primer binding site
     primer_bind     3019..3038
                     /label=Verification forward (VF2) primer binding site
     misc_feature    3151..3151
                     /label=Ambiguity; should be a T
     misc_feature    211..3158
                     /label=BBa_J61002
ORIGIN
        1 agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc
       61 gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg tcattgcgga
      121 caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgataat aaaacgtagc
      181 gtggcgatca tcgcgatact gttactagag tactagtgaa agaggagaaa tactagatgg
      241 cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg
      301 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc
      361 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt
      421 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact
      481 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg
      541 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag
      601 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg
      661 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca
      721 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca
      781 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca
      841 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact
      901 ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
      961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1021 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     1081 actagaagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3001 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3061 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     3121 ctaaggatga tttctggaat tcgcggccgc atctagag
//