##gff-version 3
##sequence-region BBa_K136040 1 3158
BBa_K136040	.	promoter	1	202	.	+	0	ID=BBa_K136006;Name=BBa_K136006
BBa_K136040	.	start_codon	138	138	.	+	0	ID=annotation1986989;Name=transcription initiation;Parent=BBa_K136006
BBa_K136040	.	non_covalent_binding_site	63	78	.	+	0	ID=annotation1986986;Name=flhDC binding site ;Parent=BBa_K136006
BBa_K136040	.	start_codon	160	162	.	+	0	ID=annotation1986988;Name=ATG;Parent=BBa_K136006
BBa_K136040	.	non_covalent_binding_site	89	104	.	+	0	ID=annotation1986987;Name=flhDC binding site;Parent=BBa_K136006
BBa_K136040	.	ribosome_entry_site	146	154	.	+	0	ID=annotation1999347;Name=nautral flgA RBS;Parent=BBa_K136006
BBa_K136040	.	plasmid_vector	211	3158	.	+	0	ID=BBa_J61002;Name=BBa_J61002
BBa_K136040	.	sequence_feature	1088	1095	.	+	0	ID=annotation1975718;Name=NotI;Parent=BBa_J61002
BBa_K136040	.	primer_binding_site	1158	1177	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site;Parent=BBa_J61002
BBa_K136040	.	sequence_feature	1095	1100	.	+	0	ID=annotation1975717;Name=PstI;Parent=BBa_J61002
BBa_K136040	.	sequence_feature	1262	1881	.	+	0	ID=annotation1975714;Name=pBR322 origin;Parent=BBa_J61002
BBa_K136040	.	ribosome_entry_site	219	230	.	+	0	ID=annotation1975766;Name=RBS;Parent=BBa_J61002
BBa_K136040	.	sequence_alteration	3151	3151	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T;Parent=BBa_J61002
BBa_K136040	.	CDS	237	917	.	+	0	ID=annotation1892273;Name=mRFP;Parent=BBa_J61002
BBa_K136040	.	sequence_feature	1259	1941	.	+	0	ID=annotation1892275;Name=ColE1 origin;Parent=BBa_J61002
BBa_K136040	.	sequence_feature	3137	3158	.	+	0	ID=annotation1975715;Name=BioBrick prefix;Parent=BBa_J61002
BBa_K136040	.	CDS	2036	2896	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_J61002
BBa_K136040	.	stem_loop	962	1005	.	+	0	ID=annotation1975713;Name=rrnB terminator;Parent=BBa_J61002
BBa_K136040	.	promoter	2938	2966	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_J61002
BBa_K136040	.	sequence_feature	212	217	.	+	0	ID=annotation1975716;Name=SpeI;Parent=BBa_J61002
BBa_K136040	.	primer_binding_site	3019	3038	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61002
BBa_K136040	.	engineered_region	219	230	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS;Parent=BBa_J61002
BBa_K136040	.	engineered_region	951	1079	.	+	0	ID=annotation1892274;Name=BBa_B0015;Parent=BBa_J61002
>BBa_K136040
agcatatctcctccgcaggtatcaaaattctgccatcagcttaaatgcctttacgcgcgcgttatcggcggaataaacg
caaaatgggtcgctatttatgccgttgatggtcattgcggacaggtacaattcacgttgtagaaatggctgggggcgaa
aatgctgataataaaacgtagcgtggcgatcatcgcgatactgttactagagtactagtgaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcc
tcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttat
ccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgc
gttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaac
ccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctta
ccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt
gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactat
cgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggt
atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgc
tctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt
ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctg
acgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttt
aaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt
gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatac
gggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaat
aaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc
cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcac
gctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaa
aaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggca
gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattct
gagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttt
aaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatg
taacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggc
aaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaag
catttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgc
acatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatca
cgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcatctagag