LOCUS       BBa_K136037              880 bp    DNA     linear       27-Dec-2024
DEFINITION  fliL promoter + WT RBS (K136009) - GFP (E0040)
ACCESSION   BBa_K136037
VERSION     BBa_K136037.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     CDS             161..880
                     /label=GFP protein
     CDS             161..880
                     /label=BBa_E0040
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag atgcgtaaag gagaagaact
      181 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      241 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      301 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      361 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      421 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      481 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      541 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      601 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      661 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      721 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      781 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      841 tgggattaca catggcatgg atgaactata caaataataa
//
LOCUS       BBa_K136043              880 bp    DNA     linear       27-Dec-2024
DEFINITION  pFliL(RBS WT)-GFP
ACCESSION   BBa_K136043
VERSION     BBa_K136043.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     CDS             161..880
                     /label=GFP protein
     CDS             161..880
                     /label=BBa_E0040
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag atgcgtaaag gagaagaact
      181 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      241 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      301 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      361 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      421 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      481 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      541 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      601 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      661 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      721 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      781 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      841 tgggattaca catggcatgg atgaactata caaataataa
//