LOCUS       BBa_K136054             1127 bp    DNA     linear       22-Dec-2024
DEFINITION  flgA promoter + WT RBS (K136006) - YFP tripart + AAV (E0434)
ACCESSION   BBa_K136054
VERSION     BBa_K136054.1  
FEATURES             Location/Qualifiers
     misc_feature    63..78
                     /label=flhDC binding site 
     misc_feature    89..104
                     /label=flhDC binding site
     misc_feature    160..162
                     /label=ATG
     misc_feature    138..138
                     /label=transcription initiation
     RBS             146..154
                     /label=nautral flgA RBS
     promoter        1..202
                     /label=BBa_K136006
     misc_feature    215..218
                     /label=conserved
     RBS             211..222
                     /label=BBa_B0034
     misc_feature    985..990
                     /label=stop
     misc_feature    946..984
                     /label=AAV
     misc_feature    229..990
                     /label=BBa_E0034
     stem_loop       1010..1053
                     /label=stem_loop
     misc_feature    999..1078
                     /label=BBa_B0010
     terminator      999..1078
                     /label=BBa_B0010
     stem_loop       1094..1113
                     /label=T7 TE
     misc_feature    1120..1120
                     /label=stop
     polyA_site      1114..1127
                     /label=polya
     misc_feature    1087..1127
                     /label=BBa_B0012
     terminator      1087..1127
                     /label=BBa_B0012
ORIGIN
        1 agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc
       61 gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg tcattgcgga
      121 caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgataat aaaacgtagc
      181 gtggcgatca tcgcgatact gttactagag aaagaggaga aatactagat ggtgagcaag
      241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      361 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      421 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
      481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      661 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      781 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
      841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
      961 gacgaaaact acgctgctgc tgtttaataa tactagagcc aggcatcaaa taaaacgaaa
     1021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//